Re: HER2 immunostaining

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From:"Sharon F. Vogt" <> (by way of histonet)
To:histonet <>
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>We use the antibody at a dilution of 1:20,000 overnight with LSAB+
>detection (DAKO) and DAB+ (DAKO) chromogen.  The antibody can be diluted
>1:2,000 for 30' incubations.

> Please keep in mind that this is an old lot
>that may not be available anymore.

Dr. Cartun gave the appropriate warning.
I was checking on availability earlier today of the DAKO c-erb-B2
concentrate. The product number previously available in a 1 mL size is
now only offered in a 0.2 mL size and has been reformulated, i.e. the
protein concentration has been changed. As I understand it, the 0.2 mL
size will supply as many tests as the 1 mL size did. That basically means
a 5-fold decrease in dilution, which would take us down to 1:400 for 30"


Sharon F. Vogt, MT (ASCP)
Pathology/Special Oncology
Dekalb Medical Center
2701 N. Decatur Road
Atlanta, GA 30033

PH:  404-501-5253
FAX: 404-297-0444

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