Beth,

more information please.  when you "stain" do you mean an enzyme
histochemical reaction or an immunohistochemical procedure?  If you are
performing the enzyme reaction using x-gal, i do not understand why you
must use an aqeous mountant.  i have always used alcohol and xylene before
mounting in a synthetic mountant and have used either eosin or nuclear fast
red as the counterstain.  if you are performing and immuno procedure,
please relate what chromagen you are using.

rob

At 03:09 PM 11/24/98 -0700, you wrote:
>Histonetters:
>
>We are "staining" frozen sections of B-gal. cells to end up with a
>turquoise-blue reaction product.  The color is soluble in alcohols and
>clearants.  We use crystal mount to permanently mount.  We are looking for
>a counterstain that will not obscure the turquoise-blue color and will not
>leach out into the aqueous mounting media.  So far our hematoxylin (Mayers)
>is too dark, and neutral and nuclear fast red bleed out into the mountant.
>Anyone have any suggestions?  I sort of remember discussions of this nature
>in the past but , of course, didn't archive them at the time.
>
>Thanks for the help,
>
>Beth Roche
>Gore Hybrid Technologies
>Flagstaff, AZ
>broche@wlgore.com
>