RE: bone marrow trephines

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From:"Weems, Joyce" <> (by way of histonet)
To:histonet <>
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We "demineralize" this way also, and have and alk phos immuno procedure
works better on our bone marrows. J:>)

>From: 	Hagerty, Marjorie A.[]
>Sent: 	Thursday, November 12, 1998 2:58 PM
>To: 	Histonet
>Subject: 	bone marrow trephines
>We are currently using RDO for one hour to decal our BM core bxs. This works
>perfectly for us, they are easy to cut, and the nuclear detail is excellent.
>I think, however, that the HCL in the decalcifier may be interfering with
>our Kappy/Lambda immunoperoxidase staining. I tried an antigen retrieval
>solution specifically for decalcified tissue but that did not seem to
>correct the problem. Doing this on one case does not count as any kind of
>"study" so this may be an excellent product. We would like to try a non-HCL
>decalcifier. Alex metioned formal citrate but our clinicians would not
>tolerate the length of time it takes. Does anyone know of a decalcifier that
>takes no more than an hour for very small core bxs and does not use HCL?
>EMC, Rancho Mirage, CA

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