RE: antibody or ? search
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From: | "Kellar, Eric" <kellarec@MSX.UPMC.EDU> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Cindy:
About the most reliable technique is the enzyme histochemical method:
Leder's Napthol AS-D chloroacetate esterase (CAE). The enzyme remains
detectable after routine formalin fixation and paraffin embedding. The stain
is (+) for mast cells and granulocytes. It is (-) for other lymphoid cells.
Immunohistochemically, immature canine myeloid cells are CD32(-),
CD64(weakly +) and with maturation, as in humans, the CD64 surface antigen
is lost and CD32 is gained. A polyclonal myeloperoxidase may also work on
canine tissue.
Eric Kellar
Histology/Immunohistochemistry
University of Pittsburgh Medical Center
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From: Cindy Farman [SMTP:cfarman@sierrabiomedical.com]
Sent: Monday, November 23, 1998 4:55 PM
To: 'Histonet'
Subject: antibody or ? search
Hi everyone,
Okay, here's a tough one. I might just have to give a prize out if
someone
can help me with this. I trying to figure out a way to detect
immature
granulocytes in formalin-fixed, paraffin-embedded dog liver.
Cindy Farman
Sierra Biomedical, Inc.
Sparks, NV
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