RE: antibody or ? search

<< Previous Message | Next Message >>
From:"Kellar, Eric" <kellarec@MSX.UPMC.EDU> (by way of histonet)
To:histonet <>
Content-Type:text/plain; charset="us-ascii"


About the most reliable technique is the enzyme histochemical method:
Leder's Napthol AS-D chloroacetate esterase (CAE). The enzyme remains
detectable after routine formalin fixation and paraffin embedding. The stain
is (+) for mast cells and granulocytes. It is (-) for other lymphoid cells.

Immunohistochemically, immature canine myeloid cells are CD32(-),
CD64(weakly +) and with maturation, as in humans, the CD64 surface antigen
is lost and CD32 is gained. A polyclonal myeloperoxidase may also work on
canine tissue.

Eric Kellar
University of Pittsburgh Medical Center

	From:  Cindy Farman []
	Sent:  Monday, November 23, 1998 4:55 PM
	To:  'Histonet'
	Subject:  antibody or ? search

	Hi everyone,

	Okay, here's a tough one. I might just have to give a prize out if
	can help me with this. I trying to figure out a way to detect
	granulocytes in formalin-fixed, paraffin-embedded dog liver.

	Cindy Farman
	Sierra Biomedical, Inc.
	Sparks, NV

<< Previous Message | Next Message >>