Histonetters:

We are "staining" frozen sections of B-gal. cells to end up with a
turquoise-blue reaction product.  The color is soluble in alcohols and
clearants.  We use crystal mount to permanently mount.  We are looking for
a counterstain that will not obscure the turquoise-blue color and will not
leach out into the aqueous mounting media.  So far our hematoxylin (Mayers)
is too dark, and neutral and nuclear fast red bleed out into the mountant.
Anyone have any suggestions?  I sort of remember discussions of this nature
in the past but , of course, didn't archive them at the time.

Thanks for the help,

Beth Roche
Gore Hybrid Technologies
Flagstaff, AZ
broche@wlgore.com