ki-67 protocol

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From:carnek@dianon.com (Kathy-Jean Carney) (by way of histonet)
To:histonet <histonet@magicnet.net>
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Does anyone have a protocol for Ki-67 (Zymed or Immunotech antibodies)
when tissue is fixed in Prefer?  Our Immuno's are done by automation.  We
do not antigen retrieve with prefer, but is there something else we can do?
We are getting all negative staining and I know some should be positive.
Thanks
Kathy




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