human frozen tissue
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From: | Patricia Landry <aries@a.imap.itd.umich.edu> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Dear Histonetters, All the recent messages about gloves and frozen
sections as well as infection control has been very interesting and
helpful. I am beginning a pilot study in which I will be working with
unfixed human arteries. A lot of my questions have already been answered.
However, I would like to know after cutting the sections, how do I "fix"
them on the slides prior to H&E and IHC?
I have heard/read that you use alcohol/acetone, but what % - 50/50? Can I
just dip them in a staining dish or do they have to lie flat? What about
time? I use superfrost/plus charged slides and have good results with my
frozen sections sticking. Then do I just rinse in dHOH and proceed as
usual? Thanks in advance for any responses.
Pat Landry
U of Michigan/ Dental
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