i had such an overwhelming response asking for this reference i'm just going
to post it here for all.

Andrew SM, Jasani B (1987) Histochem J   19:426-30

best of luck to all who try it....i think it's a great protocol for use on
frozens.  it does take a bit of time....you incubate for 1hr. @ 37C.

carrie kyle-byrne
eire@teleport.com