eosin staining problem

<< Previous Message | Next Message >>
From:Gayle Callis <uvsgc@msu.oscs.montana.edu> (by way of histonet)
To:histonet <histonet@magicnet.net>
Content-Type:text/plain; charset="us-ascii"

Could you elaborate more, please, on exactly how do your staining, from
deparaffinizing etc.

A workshop from Jerry Fredenburgh taught us some good H&E staining
hints.  After the bluing step, be sure to rinse adequately not just
to remove the bluing agent but adjust the pH (less alkaline) of t tissue
in preparation for the eosin step.  1 minute or longer with running
tap water, then he suggested an alcohol (60- 70%) BEFORE going into
the eosin.  I noticed our eosin staining was better after these
two suggestions.  Careful to deparaffinize adequately and make sure there
is not tons of paraffin carryover in the first alcohols also.

Gayle Callis

<< Previous Message | Next Message >>