eosin staining problem

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From:Ian Montgomery <ian.montgomery@bio.gla.ac.uk> (by way of histonet)
To:histonet <histonet@magicnet.net>
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>Date: Thu, 12 Nov 1998 13:03:31 -0700
>From: Gayle Callis <uvsgc@msu.oscs.montana.edu>
>Subject: eosin staining problem
>To: histonet@pathology.swmed.edu
>
>in preparation for the eosin step.  1 minute or longer with running
>tap water, then he suggested an alcohol (60- 70%) BEFORE going into
>the eosin.  I noticed our eosin staining was better after these
>two suggestions.

Gayle,
	Interesting tips
	Aqueous or alcoholic Eosin.
	Was it alcohol - Eosin or alcohol - water - Eosin.


Dr. Ian Montgomery,
West Medical Building,
University of Glasgow,
Glasgow,
G12 8QQ,
Scotland.
Tel: 0141 339 8855 Extn. 6602.
Fax: 0141 330 4100.
e-mail: ian.montgomery@bio.gla.ac.uk




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