eosin staining/Ian
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From: | Gayle Callis <uvsgc@msu.oscs.montana.edu> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Sorry, more specifically, I use Richard Allan alcoholic eosin, and
one should match the concentration of alcohol (I use ethanol) of the
eosin. If the eosin is in 60% alcohol, I would put 60% ethanol before
the eosin.
I have used aqueous eosin staining, ala Culling!, stained in aq eosin,
then differentiated the eosin with running tap water. This gave a
beautiful, delicate eosin (not the blood red sky variety, which he
disliked intensely!) that had 5 shades of color. Culling felt this
gave correct tinctoral properties to collagen vs cytoplasm vs red cells,
etc. Nothing like learning from a "master of staining techniques".
After the differentiation step, dehydration was carried out as it is
post alc eosin technic.
Gayle Callis
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