eosin staining/Ian

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From:Gayle Callis <uvsgc@msu.oscs.montana.edu> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Sorry, more specifically, I use Richard Allan alcoholic eosin, and
one should match the concentration of alcohol (I use ethanol) of the
eosin.  If the eosin is in 60% alcohol, I would put 60% ethanol before
the eosin.

I have used aqueous eosin staining, ala Culling!, stained in aq eosin,
then differentiated the eosin with running tap water.  This gave a
beautiful, delicate eosin (not the blood red sky variety, which he
disliked intensely!) that had 5 shades of color.  Culling felt this
gave correct tinctoral properties to collagen vs cytoplasm vs red cells,
etc.  Nothing like learning from a "master of staining techniques".

After the differentiation step, dehydration was carried out as it is
post alc eosin technic.

Gayle Callis

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