decalcification prior immunostaining

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Dear members,

I would like to perform immunohistostaining on whole mice thorax sections.
I have to do decalcification of these thorax to make the sections. I used
the RDO decalcifier which works perfectly.
If anyone has any suggestions which could help me to perform the
immunostaining after a decalcification step, I will be greatful.


Ayadi Abdelkader
Institut de Genetique et de Biologie Moleculaire et Cellulaire (IGBMC)
BP 163
67404 Illkirch cedex FRANCE

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