cartilage
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From: | NTT@shcc.org (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Hi,
I know I've posted this in the past, but does anyone have a good technique for
sectioning of frozens or paraffin embedded cartilage. I know about the
CryoJane, but my boss is not will to spend the $6000. Or if anyone has
cartilage sections that they're willing to share, I'd love to hear from you.
It can be human or bovin (prefer human). Or if you know of a vendor that
sells cartilage sections, please let me know.
I can section my own, but I always get a wrinkle in the middle of the section.
Unfortunately, to publish our results, I need wrinkle free slides. I am using
plus charge slides.
My other questions is "does anyone do a lot of cell fixation?" I currently fix
my cells with 4% paraformaldehye and it works fine except that it leaves a film
(grayish/green) on the slide and seems to dim my fluorescence results. I'm
down to my last step to publish the result, but I can't get a good picture on
my cells because of this problem. I'd appreciate any help or suggestions.
If you want, you can respond to me directly at NTT@shcc.org.
Thanks,
Noi
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