TEM - embryos

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From:Jane Chambers <jane@cc.usu.edu> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Hi,

My question pertains to transmission electron microscopy (TEM) on mammilian
embryos.  I have done a literature search and will be getting copies of
articles soon.  However, I was wondering if anyone out in Histoland has
processed mammilian embryos for TEM?  From the materials and methods of the
articles I have seen, they are fixed in standard EM fixative, rinsed, fixed
with OsO4, rinsed, dehydrated, and embedded in Epon - all similiar to what I
am used to.  Do you use standard BEEM capsules?

Any help will be greatly appreciated,

Thanks,
Jane


M. Jane Chambers, MS, LAT
Research Technician
USDA/ARS
Poisonous Plant Research Lab
1150 East 1400 North
Logan, UT  84341
Phone:  (435) 752-2941
FAX:  (435) 753-5681
jane@cc.usu.edu




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