TEM - embryos
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From: | Jane Chambers <jane@cc.usu.edu> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
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Hi,
My question pertains to transmission electron microscopy (TEM) on mammilian
embryos. I have done a literature search and will be getting copies of
articles soon. However, I was wondering if anyone out in Histoland has
processed mammilian embryos for TEM? From the materials and methods of the
articles I have seen, they are fixed in standard EM fixative, rinsed, fixed
with OsO4, rinsed, dehydrated, and embedded in Epon - all similiar to what I
am used to. Do you use standard BEEM capsules?
Any help will be greatly appreciated,
Thanks,
Jane
M. Jane Chambers, MS, LAT
Research Technician
USDA/ARS
Poisonous Plant Research Lab
1150 East 1400 North
Logan, UT 84341
Phone: (435) 752-2941
FAX: (435) 753-5681
jane@cc.usu.edu
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