TEM - embryos

<< Previous Message | Next Message >>
From:Jane Chambers <jane@cc.usu.edu> (by way of histonet)
To:histonet <histonet@magicnet.net>
Content-Type:text/plain; charset="us-ascii"


My question pertains to transmission electron microscopy (TEM) on mammilian
embryos.  I have done a literature search and will be getting copies of
articles soon.  However, I was wondering if anyone out in Histoland has
processed mammilian embryos for TEM?  From the materials and methods of the
articles I have seen, they are fixed in standard EM fixative, rinsed, fixed
with OsO4, rinsed, dehydrated, and embedded in Epon - all similiar to what I
am used to.  Do you use standard BEEM capsules?

Any help will be greatly appreciated,


M. Jane Chambers, MS, LAT
Research Technician
Poisonous Plant Research Lab
1150 East 1400 North
Logan, UT  84341
Phone:  (435) 752-2941
FAX:  (435) 753-5681

<< Previous Message | Next Message >>