TEM - embryos

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From:Ian Montgomery <ian.montgomery@bio.gla.ac.uk> (by way of histonet)
To:histonet <histonet@magicnet.net>
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>Date: Tue, 10 Nov 1998 09:02:34 -0600 (MDT)
>From: Jane Chambers <jane@cc.usu.edu>
>Subject: TEM - embryos
>To: histonet@pathology.swmed.edu
>MIME-version: 1.0
>My question pertains to transmission electron microscopy (TEM) on mammilian
>embryos.  I have done a literature search and will be getting copies of
>articles soon.  However, I was wondering if anyone out in Histoland has
>processed mammilian embryos for TEM?  From the materials and methods of the
>articles I have seen, they are fixed in standard EM fixative, rinsed, fixed
>with OsO4, rinsed, dehydrated, and embedded in Epon - all similiar to what I
>am used to.  Do you use standard BEEM capsules?
>Any help will be greatly appreciated,

	 Fixation, your route will be glutaraldehyde followed by
postfixation with osmium. The buffer, whatever looks good, but watch the
osmolarity of the chosen buffer. Try what others in the field have used,
why re-invent the wheel.
	What you use for embedding is entirely specimen dependent. If you
need specific orientation, flat embed, but if it's just a wee lump, Beem
capsules will be just fine.

Dr. Ian Montgomery,
West Medical Building,
University of Glasgow,
G12 8QQ,
Tel: 0141 339 8855 Extn. 6602.
Fax: 0141 330 4100.
e-mail: ian.montgomery@bio.gla.ac.uk

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