Superfrost plus..large slides -Reply

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From:Sarah Christo <schristo@CVM.TAMU.EDU> (by way of histonet)
To:histonet <>
Content-Type:text/plain; charset="us-ascii"

Dear Trisha,
   This is from JOH Vol.2, No. 4, Dec 1988,
Unger, Automation of Insitu Hybridization.
1.  Place slides in a plastic rack
2.  acetone 2 minutes, drain slides
3.  2% aminopropyltriethoxsilane (Sigma #A3648)
4.  3 dishes of DI water, 5 dips each (I only do
one dish 10 dips)
5.  drain and dry in oven at 60-80 deg. C. or
note: the silane turns brown with time and will
make the slides have an amber background if
you use it that way.   -Sarah, Texas A&M
PS, I watched Frankenweenie over the weekend,
Its about a dog named Sparkie that gets killed by
a car and the little boy that owns him brings him
back to life in the attic using all that great
electricity.  I've heard of another lab getting
sparks and fires going with this procedure if you
use metal racks.  I still use them, but I like to
live dangerously.

Hi Netters,

I have been using superfrost plus slides for brdu work.  Now I have to
make larger sections of the tmj that will not fit on the 3x1 slides.  Are
there any larger slides with the coating and charge being made out there?
If not, how do I coat them and/or charge them myself?  (I am seeing the
old Frankenstein movie with lightning and cables as I write this.
Halloween had its effect I guess.)

Thanks for your time.

Trisha Emry
U of Washington, Seattle

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