Re: staining neurons in mouse cell culture

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From:Nora Fox <nefox@leland.Stanford.EDU> (by way of histonet)
To:histonet <histonet@magicnet.net>
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What about using an anti-NCAM antibody?  Good luck.

>Good Afternoon All,
>Has anyone out there in Histoland ever done staining on mouse cell cultures
>to visualize neurons? The cells will be fixed in 1% Paraformaldehyde in
>DPBS. I am in a quandary as to what Antibody to try, this is our first run
>through, any thoughts?
>Thank you in advance for any help!
>Mary Georger
>Astra Arcus

Nora Fox
Division of Orthopaedic Surgery
Stanford University
Edwards R144, MC 5341
300 Pasteur Drive
Stanford, CA 94305
phone: (650)725-2746
fax:  (650)723-6396




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