Re: sectioning epon

<< Previous Message | Next Message >>
From:Tim Morken <> (by way of histonet)
To:histonet <>
Content-Type:text/plain; charset="us-ascii"


You would certainly get better results with a glass knife. Why is this
being done?

You could try mounting the sections on a slide in epon with a
coverglass. Of course, you would have to oven-cure overnight.

Is this tissue fixed with osmium? That would make H&E staining
impossible (it would stain,but you can't tell because the osmum hides
it). Even without osmium you won't get the same colors with an H&E on
epon sections as you will in GMA or paraffin sections. Toluidine blue is
usually used on epon sections.

Tim Morken, B.S., EMT(MSA), HTL(ASCP)
Infectious Disease Pathology
Centers for Disease Control
1600 Clifton Rd.
Atlanta, GA 30333


FAX:  (404)639-3043

----Original Message Follows----
Date: Wed, 11 Nov 1998 16:46:00 -0800
From: Cathy Mayton <>
Subject: sectioning epon

Fellow histonetters,

I have been trying to section epon blocks containing an artery with a
tungsten-carbide knife.  My sections appear cloudy at 3-6 microns.  Does
epon have to sectioned thinner than 3 um and can it only be sectioned
glass knives.

Also, what is the best way to dry the sections.  So far the literature I
have says room temp over night.  What is the concensus?  I also gleened
from the literature that H&E cannot be done on epon sections.

The block polymerized well and contains no bubbles.  The block is
sectioning easily other than the sections appear cloudy and not clear
GMA or MMA sections.  Any help would be appreciated.

Cathy A. Mayton
Wasatch Histo Consultants, Inc.

Get Your Private, Free Email at

<< Previous Message | Next Message >>