Re: ki-67 protocol
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From: | Katri Tuomala <katri@istar.ca> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Daisey Joseph wrote:
>
> Hi Histonetters:
> We have tried everything from different pretreatment protocols to
>different concentrations of the antibody and have concluded that antigen
>Ki67 antigen is masked by the Prefer fixative. I have been in contact
>with other labs using Prefer fixative and the experience is mutual. If
>someone out there has found a way to make it work, I'd like to know also.
>
> Daisy Joseph
> Impath Inc.
>
> -----Original Message-----
> From: Kathy-Jean Carney [SMTP:carnek@dianon.com]
> Sent: Tuesday, November 10, 1998 7:16 AM
> To: 'Histonetters'
> Subject: ki-67 protocol
>
> Does anyone have a protocol for Ki-67 (Zymed or Immunotech antibodies)
>when tissue is fixed in Prefer? Our Immuno's are done by automation. We
>do not antigen retrieve with prefer, but is there something else we can
>do? We are getting all negative staining and I know some should be
>positive. Thanks
> Kathy
Hi there!
It seems to me that this Prefer fixative simply does not preserve this
particular antigen. I don't know what is in Prefer (trade secret), but
many so called formalin substitutes are alcohol based and some antigenic
epitopes are not preserved by alcohol.
Just a thought! Katri.
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