According to Lee Luna's book,  Glycerin can be used in the same proportion.
Omission of chloral hydrate will not effect the staining properties or life
of the stain.

I can fax you this info if you send me your fax number.

Rande Kline HT (ASCP)
Technical Services
EM Science







"Carpenter, Judith A." <Jude.Carpenter@vtmednet.org> on 11/02/98 12:34:31
PM

To:   'NSH' <Histonet@pathology.swmed.edu>
cc:
Subject:  chloral hydrate





Dear fellow Histonetters-
Does anyone know if chloral hydrate is absolutely necessary to the making
of
Mayer's
Hematoxylin ?  Can it be substituted with something not requiring a DEA
(Drug Enforcement Agency) number ?
We make our hematoxylin (1 liter) up weekly so it doesn't sit long enough
to
"go bad". If we
filter it daily will we be okay w/o the chloral hydrate (it supposedly
reduces scum/precipitate formation) ?
The reasons we don't buy commercial hematoxylin involve the subjective
opinions of 19 pathologists
and numerous trials of other products over the years.  Our "home brew" is
what they like best and
is incredibly cheaper than other products.
Thanks for your help !!
Jude
Jude Carpenter,BS, HTL(ASCP)
FAHC/MCHV Campus
111 Colchester Ave.
Burlington,   VT  05401
(802)656-5116
jude.carpenter@vtmednet.org