Re: chloral hydrate

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From:Mick Rentsch <> (by way of histonet)
To:histonet <>
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Dear Judith,
the Chloral Hydrate is only there to act as an antioxidant/preservatve and
to prevent bacterial growth. You can eliminate it from your recipe and
substitute Glycerol BP up to 30%. Using 30 % conc. Glycerol you can expect
an unopened shelf life of between 1-2yrs, it will discourage evaporation and
is non-toxic. To get maximum shelf life out of your Mayer's, do not go over
0.2g/L with either Sodium or Potassium Iodate.
You may find that some agitation during staining may be helpful.
Regards Mike Rentsch (Downunder)
-----Original Message-----
From: Carpenter, Judith A. <>
To: 'NSH' <>
Date: Tuesday, 3 November 1998 5:22
Subject: chloral hydrate

>Dear fellow Histonetters-
>Does anyone know if chloral hydrate is absolutely necessary to the making
>Hematoxylin ?  Can it be substituted with something not requiring a DEA
>(Drug Enforcement Agency) number ?
>We make our hematoxylin (1 liter) up weekly so it doesn't sit long enough
>"go bad". If we
>filter it daily will we be okay w/o the chloral hydrate (it supposedly
>reduces scum/precipitate formation) ?
>The reasons we don't buy commercial hematoxylin involve the subjective
>opinions of 19 pathologists
>and numerous trials of other products over the years.  Our "home brew" is
>what they like best and
>is incredibly cheaper than other products.
>Thanks for your help !!
>Jude Carpenter,BS, HTL(ASCP)
>111 Colchester Ave.
>Burlington,   VT  05401

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