P. Emry wrote:
>
> Hi Peggy,
> This looks like it will help.  Do you find a difference between ETOH and
> Isoprop. alcohol?

Never tried it with IsOH, but don't see why it wouldn't work.
Try it, and let us know.

> Thanks,
> Trisha
>
> On Wed, 4 Nov 1998, Wenk, Lee & Peggy wrote:
>
> > NTT@shcc.org wrote:
> > >
> > > Hi,
> > >
> > > I know I've posted this in the past, but does anyone have a good
>technique for
> > > sectioning of frozens or paraffin embedded cartilage.
> >
> > (I know the following works for paraffin. Have never tried it with FS.)
> >
> > Have you ever tried floating the tissue out on 25% alcohol, then
> > transferring it to the flotation bath?
> >
> > We take a blank slide (frosted end ones OK), lay it flat on
> > the counter, and flood it from a squirt bottle with 25% alcohol.
> >
> > Then lay the tissue on the alcohol.
> >
> > Carefully drain off the excess alcohol onto a paper towel by
> > lifting one corner of the slide and holding onto the tissue.
> >
> > Then slowing lower your slide into the water bath at a slight
> > angle. The wrinkles/folds should be parallel to the edge of
> > water coming up the slide. In other words, you can put the
> > slide in bottom first or side first or diagonally. Just so
> > that the wrinkles are parallel to the surface of the water,
> > not perpendicular.
> >
> > The difference in surface tension should put a little more
> > "pull" on the section, straightening out more of the
> > wrinkles, folds. The sections might spin around due to this
> > surface tension, so watch it if you have to maintain the
> > same orientation slide after slide. The more alcohol you drain
> > off, the less spinning, but also less pulling. So play around
> > with the amount you need drained off.
> >
> > You can do this with one section, or a ribbon, as long as
> > it fits on the slide.
> >
> > About the only difference I have ever seen between this and
> > slides that I did NOT float on the alcohol is that those with
> > the alcohol might stain a SLIGHTLY lighter shade with the
> > eosin. Nothing noticable, unless you were doing serial sections,
> > some with and some without alcohol, and then laid them
> > next to each other in a folder and LOOKED for the difference.
> > Microscopically, they look the same.
> >
> > I'd slide with the subbed slides.
> >
> > Hope this helps.
> >
> > > CryoJane, but my boss is not will to spend the $6000. Or if anyone has
> > > cartilage sections that they're willing to share, I'd love to hear
>from you.
> > > It can be human or bovin (prefer human).   Or if you know of a vendor
>that
> > > sells cartilage sections, please let me know.
> > >
> > > I can section my own, but I always get a wrinkle in the middle of the
>section.
> > > Unfortunately, to publish our results, I need wrinkle free slides.  I
>am using
> > > plus charge slides.
> > >
> > > .
> > >
> > > Thanks,
> > > Noi
> >
> > --
> > Peggy A. Wenk, HTL (ASCP)
> > Anatomic Pathology
> > Wm. Beaumont Hospital
> > 3601 W. 13 Mile Rd.
> > Royal Oak, MI 48073-6769
> >
> >

--
Peggy A. Wenk, HTL (ASCP)
Anatomic Pathology
Wm. Beaumont Hospital
3601 W. 13 Mile Rd.
Royal Oak, MI 48073-6769