Re: Tissue Prep

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From:Mick Rentsch <ausbio@nex.com.au> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Dear Joyce,
either the cause is the presence of low MW paraffins or Xylenes like you
suspect, or their blend has a melting point of less than 56C.
We had this problem with Tissue prep. about 18 years ago almost Australia
wide, and after not getting much response from the Importers, the Histo
Discussion Groups also raised the issue, and most persons discontinued the
use of Tissue Prep. and changed to either one of the three following :-
Paramat (BDH/Merck/EM Scientific), Paraplast (Sherwood Industries). A few
labs also swore by Paraplast Plus, but most of us elected not to use a
product that contained DMSO that might encourage skin absorbtion of other
lab nasties (Maybe that's not a problem with the correct gloves).
In your shoes I'd get in one of the other waxes immediately, then when you
are confident Tissue Prep have fixed the problem, go back to it if you think
it's wise.
Regards Mike Rentsch (Downunder)
-----Original Message-----
From: Joyce Friedland <joycefr@frontiernet.net>
To: histonet@Pathology.swmed.edu <histonet@Pathology.swmed.edu>
Date: Friday, 13 November 1998 4:19
Subject: Tissue Prep


>Hi All,
>Is anyone else having problems with Tissue Prep paraffin they have received
>in the past two weeks?  Our sections are "exploding" in a way similar to
>blocks that are comtaminated with xylene. As a test we tried cutting some
>plain paraffin blocks (no tissue) and saw the same problem. Lowering the
>waterbath temp. helps only a bit.
>Any ideas??
>What other paraffin brands are people using?
>
>Thanks, Joyce
>
>************************************
>*  Joyce Friedland                 *
>*  joycefr@frontiernet.net         *
>*  www.frontiernet.net/~joycefr    *
>************************************
>
>
>




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