Re: Thin Brain sections (fwd)

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From:Ian Montgomery <> (by way of histonet)
To:histonet <>
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>Dear Kim,
>I would suggest that you wrap your tissue in histowrap or some type of lens
>paper then place between sponges to keep flat. You don't want to just place
>between sponges or you will get an artifact that will also cause you
>problems. I would also suggest that you get rid of any heat in any of your
>processing stations except for the paraffins. Also, how long are you in
>paraffin sometimes the heat from the paraffin causes the tissue to curl so
>if you decrease the time in the paraffin this will help.
>Hope this is helpful. If you have further questions or need further
>assistance please let me know.
>Lynn Gardner, HT(ASCP)
>Supervisory Research Assistant III
>University of Iowa
>FC Blodi Eye Pathology Laboratory
>233 MRC
>Phone: 319-335-7095
>Fax: 319-335-7193

	Artifact, is it the occasional wee dimple impression from the
sponge, normally taken away when trimming, or something else.

>>	I sandwich the slices between tissue processing foam pads, place in
>>a cassette and process in the normal way.

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