Re: Superfrost plus..large slides -Reply

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From:"P. Emry" <> (by way of histonet)
To:histonet <>
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Hi Sarah,
Thanks for the information...especially the fire hazard part.  My boss
has a great sense of humor, but I don't think I will test it with sparks
etc.  But I will keep it in mind for next Halloween.


On Wed, 4 Nov 1998, Sarah Christo wrote:

> Dear Trisha,
>    This is from JOH Vol.2, No. 4, Dec 1988,
> Unger, Automation of Insitu Hybridization.
> 1.  Place slides in a plastic rack
> 2.  acetone 2 minutes, drain slides
> 3.  2% aminopropyltriethoxsilane (Sigma #A3648)
> 4.  3 dishes of DI water, 5 dips each (I only do
> one dish 10 dips)
> 5.  drain and dry in oven at 60-80 deg. C. or
> overnight.
> note: the silane turns brown with time and will
> make the slides have an amber background if
> you use it that way.   -Sarah, Texas A&M
> PS, I watched Frankenweenie over the weekend,
> Its about a dog named Sparkie that gets killed by
> a car and the little boy that owns him brings him
> back to life in the attic using all that great
> electricity.  I've heard of another lab getting
> sparks and fires going with this procedure if you
> use metal racks.  I still use them, but I like to
> live dangerously.
> Hi Netters,
> I have been using superfrost plus slides for brdu work.  Now I have to
> make larger sections of the tmj that will not fit on the 3x1 slides.  Are
> there any larger slides with the coating and charge being made out there?
> If not, how do I coat them and/or charge them myself?  (I am seeing the
> old Frankenstein movie with lightning and cables as I write this.
> Halloween had its effect I guess.)
> Thanks for your time.
> Trisha Emry
> U of Washington, Seattle

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