Re: Superfrost plus..large slides -Reply
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From: | "P. Emry" <emry@u.washington.edu> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Hi Sarah,
Thanks for the information...especially the fire hazard part. My boss
has a great sense of humor, but I don't think I will test it with sparks
etc. But I will keep it in mind for next Halloween.
Cheers,
Trisha
On Wed, 4 Nov 1998, Sarah Christo wrote:
> Dear Trisha,
> This is from JOH Vol.2, No. 4, Dec 1988,
> Unger, Automation of Insitu Hybridization.
> 1. Place slides in a plastic rack
> 2. acetone 2 minutes, drain slides
> 3. 2% aminopropyltriethoxsilane (Sigma #A3648)
> 4. 3 dishes of DI water, 5 dips each (I only do
> one dish 10 dips)
> 5. drain and dry in oven at 60-80 deg. C. or
> overnight.
> note: the silane turns brown with time and will
> make the slides have an amber background if
> you use it that way. -Sarah, Texas A&M
> PS, I watched Frankenweenie over the weekend,
> Its about a dog named Sparkie that gets killed by
> a car and the little boy that owns him brings him
> back to life in the attic using all that great
> electricity. I've heard of another lab getting
> sparks and fires going with this procedure if you
> use metal racks. I still use them, but I like to
> live dangerously.
>
>
> Hi Netters,
>
> I have been using superfrost plus slides for brdu work. Now I have to
> make larger sections of the tmj that will not fit on the 3x1 slides. Are
> there any larger slides with the coating and charge being made out there?
> If not, how do I coat them and/or charge them myself? (I am seeing the
> old Frankenstein movie with lightning and cables as I write this.
> Halloween had its effect I guess.)
>
> Thanks for your time.
>
> Trisha Emry
> U of Washington, Seattle
>
>
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