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HistoNet Server wrote:

> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 03:15:46 -0500
> From: "Ms Louise Taylor" <179LOU@chiron.wits.ac.za>
> Subject: Re: histonet solicitors poll
>
> Well, here's my 2 cents worth,
>
> If decisions on equipment purchases etc are not your decision then
> you should not be getting steamed up about vendors contacting you,
> you should just politely tell them that if necessary they should
> contact your supervisor.
> I feel that people using the histonet as a market survey tool is just
> peachy - at least they know what competition is out there and whether
> their sales  tactic and product is suitably market oriented. I have
> often suggested to vendors other possible targets for
> their product - people that i know are looking for a particular item
> or have an interest in a particualr field - I do not consider this
> inappropriate - I hope that other s will do the same for me.
>
> Life is too short to get agitated on such issues
>
> Louise taylor
>
> On 14 Oct 98 at 20:57, Mark & Carrie Byrne wrote:
>
> > hi all,
> > i'd like to get others opinions about the current debate about this.
> > here's my feeling:
> > i've no problem at all with sales and tech reps giving info or advice
>on the
> > topics that come up, just like any other histonet subscriber (hey....i
> > spelled it right!!  there's hope for me yet :-)).  however, i'd rather that
> > the line be drawn at that.  personally, i do not welcome sales pitches from
> > out of the blue.  especially since purchasing decisions are the
> > supervisor's, not mine.
> >
>
> > ok, end of diatribe from me.
> >
> > carrie kyle-byrne
> > eire@teleport.com
> >
> >
> >
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 07:30:52 -0500
> From: "Gamble,Marilyn S" <Marilyn.S.Gamble@kp.ORG>
> Subject: Head Hunters
>
> Just wanted to clarify that I am not a proponent of head hunters using the
> Histonet.  (But, they will).  The savvy ones just won't mention it anymore.
> They go to great lengths to get to people and have even called me at work to
> see if any of our employees were looking for jobs!  (That goes beyond
> nerve).  The point made was that they have no "product" to boycott.
> Apologies if my histonet friends thought I was in favor.
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 08:16:06 -0500
> From: Joanne Schoonmaker <schoonmj@VAX.CS.HSCSYR.EDU>
> Subject: Trying to locate Diane Sterchi
>
> Does anyone have a telephone number or address for Diane Sterchi?
>
> TIA,
> Joanne Schoonmaker
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 08:24:37 -0500
> From: "Lewin, Anne C." <lewina@bms.com>
> Subject: FW: Histonet Soliciters
>
> I don't usually respond to non-technical questions on the histonet, but felt
> compelled to in this case.
> I use a couple of Hacker products, and have had nothing but wonderful
> experiences with their sales and technical people.  The instruments run
> smoothly and effeciently, and I have never had a problem with them.  I hope
> that everyone re-reads this eloquent letter from Hacker and takes to heart
> that you should NOT bash an entire company for one isolated incident that may
> or may not have happened.
> As far as whether vendors/headhunters should use the histonet for soliciting
> business, I really don't mind keeping current with technologies or job
> opportunities that may find themselves on our listserv.  It actually is a
> great thing that these people take the time to listen to our questions and
> concerns and can try and improve their products to keep up with our demands
> for excellence.  True, I don't care to have an experiment interrupted for a
> sales call, and I never spend time speaking with these people (I just hang
> up), but I don't mind it on the histonet.  If I'm not interested, I just hit
> the delete key, and everyone here has that same option.
> Well, that's my two cents worth, now how about some real histology questions
> and enough of this Soliciters bashing???
>
>
>_______________________________________________________________________________
>> From: HACKERLAB@aol.com on Wed, Oct 14, 1998 4:41 PM
> Subject: Re: Histonet Soliciters
> To: eire@teleport.com
> Cc: HistoNet@Pathology.swmed.edu
>
> Dear Mark & Carrie Byrne;
>
> As the head of the head office I am responding to your e-mail, and quite
> frankly I am a bit shocked! I am sorry to hear of your aggressive sentiments
> towards Hacker, and on behalf of the same, I offer personal and corporate
> apologies if you have been in any way offended.
>
> I have checked with our direct sales people, and none of them have any record
> of speaking with you. As you may or may not know, we work with a number of
> independents nationwide and they may or may not have spoken with you
>regarding
> automated coverslipping. Most of us, who are involved with this industry are
> on the HistoNet. It is normal, and I don't think inappropriate, to expect
>some
> type of follow up when questions regarding products and their performance are
> aired over the HistoNet. Vendors do this, because it is our job to know how
> our instruments and our competitors are doing out there. Our customers are
> also one of our best source of ideas which can hopefully be used towards
> improving our products to better meet their needs. This can only happen
> through an open exchange of information.
>
> It would be very helpful, and truly appreciated, if you would provide me with
> the details regarding the incident with a Hacker Rep which you find so
> offensive. Who was the Rep; and what issue did they respond to?  What
>could be
> so reprehensible that it could incite you to vent such an aggressive reaction
> toward an entire company and question our sales practices? Believe me, if
>it's
> that bad, I certainly need to know! I might also add, that it may be more
> effective to contact "The Company", (that would me in this case), directly to
> air your views and vent your anger at someone you could hopefully give you
> some answers, rather than making inflammatory blanket statements over the
> HistoNet, don't you think?
>
> What you should know, is that Hacker has been in this business since 1942. We
> take our business rather seriously. As a company, and as individuals (many of
> whom are histotechs), we make every effort to support this industry, it's
> Societies, affiliates, etc., including the HistoNet.
>
> A rational explanation for your comments would be most welcome. Hopefully
>your
> input can help us avoid this type of situation in the future.
>
> I look forward to hearing from you at your earliest convenience.
>
> Sincerely,
>
> Elfi Hacker
> President
> HACKER Instruments & Industries Inc
>
> Tel. (973) 226-8450
> Fax.(973) 808-8281
> e-mail: HACKERLAB@AOL.COM
>
> - ------------------ RFC822 Header Follows ------------------
> Received: by msmail.bms.com with ADMIN;14 Oct 1998 16:39:12 -0400
> Received: from directory-daemon by monju.bms.com (PMDF V5.1-10 #28009)
>  id <0F0U001013W4N9@monju.bms.com> for
>  lewin#m#_anne_c#d#.prilvms1@msmail.bms.com; Wed,
>  14 Oct 1998 16:36:04 -0400 (EDT)
> Received: from swax32.swmed.edu by monju.bms.com (PMDF V5.1-10 #28009)
>  with SMTP id <0F0U0018T3VUG7@monju.bms.com> for lewina@bms.com; Wed,
>  14 Oct 1998 16:36:04 -0400 (EDT)
> Received: from 129.112.18.39 ([129.112.18.39])
>  by SWAX32.SWMED.EDU (PMDF V5.1-8 #18228)
>  with SMTP id <01J2YS4MS4428ZL76R@SWAX32.SWMED.EDU> for lewina@bms.com; Wed,
>  14 Oct 1998 15:34:03 -0600 (CST)
> Date: Wed, 14 Oct 1998 16:26:38 -0400 (EDT)
> From: HACKERLAB@aol.com
> Subject: Re: Histonet Soliciters
> To: eire@teleport.com
> Cc: HistoNet@Pathology.swmed.edu
> Message-id: <ae3e4722.362508fe@aol.com>
> MIME-version: 1.0
> Content-type: text/plain; charset=US-ASCII
> Content-transfer-encoding: 7bit
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 08:33:42 -0500
> From: "O'Neil, David B." <David.O'Neil@nrc.ca>
> Subject: LKB Knifemaker breaking pads
>
> I am in search of breaking pads for an LKB 2078 Histo Knifemaker (for Ralph
> Knives).  Any
> suggestions?
>
> David O'Neil                                                            tel:
> (902) 426-8258
> National Research Council of Canada             fax:   (902) 426-9413
> Institute for Marine Biosciences
> 1411 Oxford St.
> Halifax, Nova Scotia B3H 3Z1
> Canada
> email:  david.o'neil@nrc.ca
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 08:43:09 -0500
> From: "Alan Bright" <Bright@dial.pipex.com>
> Subject: Re:
>
> I have not sent any enclosures
>
> - -----Original Message-----
> From: HistoNet Server <HistoNet@Pathology.swmed.edu>
> To: Alan Bright <Bright@dial.pipex.com>
> Date: Thursday, October 15, 1998 12:29
>
> >Please do not send ENCLOSURES!!!!!!
> >
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 09:15:29 -0500
> From: "Liz Sabin" <esabin@srv4.med.ed.ac.uk>
> Subject: Substance P
>
> Hello all,
>
> To the person who asked for information regarding a source of
> Substance P antibody (sorry, I deleted your original message) - the
> following may be of use, although I can't remember the exact details
> of your request:
>
> BioGenex do a rabbit anti-human polyclonal substance P antibody.
> The company sells several versions of this antibody, but the one
> which is used in my lab is the "Super Sensitive Ready to Use"
> antibody (pre-diluted), catalogue no. AR069-SR.
>
> We incubate for 30 mins, following microwave antigen retrieval in
> 10mM Citrate buffer pH6 for 3x5 mins.
>
> In the UK, BioGenex products are distributed by Biomen Diagnostics
> (Tel. 0118 973 0013) and in the US I believe you can deal with
> BioGenex directly (Tel 800 421 4149).
>
> Hope this helps!
>
> Best wishes
> Liz Sabin
> Liz Sabin
> Department of Pathology
> University of Edinburgh
> Medical School
> Teviot Place
> Edinburgh
> EH8 9AG
>
> email. esabin@srv4.med.ed.ac.uk
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 09:25:23 -0500
> From: LINDA MARGRAF MD <LMARGRAF@childmed.dallas.tx.us>
> Subject: Re: enclosures
>
> Dear Alan and other Histonetters:
> This enclosure issue is coming up frequently now.
> Here's the story:"Enclosures" are the same as "attachments" or "attached
> files" and there are a number of ways that these might creep into your
> email:
> First:  Check your email program. Some like Claris Emailer and some Microsoft
> programs automatically attach little enclosures to email messages. These are
> supposed to authenticate that the message was really sent by who put their
> name on it. A security feature, but an unnecessary one. You should be able to
> delete this.
>
> Microsoft Explorer is set up to send email as an attachment unless the
> defaults are changed. (They are in the preferences, I think.)
>
> Other ideas:
>
> 1. You might have put enclosures in your mail by attaching a picture or text
> file using a menu choice like "Insert file".
> 2. There might be graphics in your email template, either in the page
> design or signature.
> 3. If you are sending using a web browser you may well be sending
> embedded files without knowing it.
> 4. If you are sending via a word processor, the output may well be bundled as
> a simple (just header and signature) email with the word
> processor document file attached to it.
>
> There are good reasons why attached files should not go over the
> histonet: they can be very large and word processor files can transmit
> macro viruses, to name but two. We=ve actually blown out sservers all over by
> having a large attachment go out over the net!
>
> Good luck!
>
> Linda M
> Histonet administrator
>
> P.S. Thanks to the subscribers who sent me some of the enclosure info in this
> message.
>
> >>> Alan Bright <Bright@dial.pipex.com> 10/15 8:31 AM >>>
> I have not sent any enclosures
>
> - -----Original Message-----
> From: HistoNet Server <HistoNet@Pathology.swmed.edu>
> To: Alan Bright <Bright@dial.pipex.com>
> Date: Thursday, October 15, 1998 12:29
>
> >Please do not send ENCLOSURES!!!!!!
> >
>
>
>
>
>
>
>
>
>
>
>
>
>
>                                          !
>
>
>
>
>
>
>
>
>
>
>
>
>
>                                          !
>
>
>
>
>
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 09:46:15 -0500
> From: tflore@lsumc.edu (Flores, Teresa)
> Subject: Re: Staining Spurr's???
>
> Hildegard, Our EM lab has been using Spurr epoxy for the past 20 years.
> Please provide your "get rid of spurr" with references. Thanks, Teresa
>
> >------------------------------------------------------------------------
> >The Microscopy ListServer -- Sponsor: The Microscopy Society of America
> >To  Subscribe/Unsubscribe -- Send Email to ListServer@MSA.Microscopy.Com
> >On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
> >-----------------------------------------------------------------------.
> >
> >
> >Hi,
> >
> >Spurr's is the most highly crosslinked of all embedding media used for
> >LM-TEM.  The higher the crosslinkage, the less likely any stain, whether
> >for LM or heavy metal for TEM, is to penetrate well enough to be called
> >successfull.
> >First:  Get rid of Spurr's.  It contains a potent carcinogen, VCD.  Do you
> >need this?
> >Second:  If you cannot get rid of Spurr's (try real hard), then use the
> >softest mixture you can tolerate for cutting.  Polymerize at 60deg C
> >overnight, and see if this is adequate.  This will reduce the final
> >crosslinkage.
> >Third:  Blocks in existence already! Don't stain well?  Try soaking the
> >sections prior to staining for extended periods of time in water, then 5
> >min in alcohol, in increasing concentrations until you reach 95%.  Then go
> >back down to water stepwise.  Try staining.
> >Fourth:  Use as alkaline as possible a vehicle for your stain.  pH 12 is
> >about right.
> >Fifth:  Combine all of the above.  Does not work?  Soak the sections in
> >water and gradually bring to 95% alcohol..  Expose to stain dissolved in
> >alcohol.  Does not work?  Forget it.  Start over.
> >
> >If you have very valuable sections and you must stain them for TEM, use
> >alcoholic UA for 10min at 60deg C.  Use Reynolds lead citrate at a pH of
> >about 9 or 10.  This last trick is truly a last resort, since the lead may
> >dump erratically (or stain easily) at this low pH.
> >
> >If you polymerize a block at a low power for 45 minutes in a microwave,
> >you crosslink the resin to such an extent that nothing, nothing, nothing
> >you do will stain it.  (Lost my best meat loaf dish this way).  For TEM we
> >do not polymerize resins totally, about 10% of the monomers are left
> >unreacted with one another.  If you "drive" the crosslinkage, the block
> >will be harder, less elastic, and impenetrable for liquids (except if you
> >boil it for a year or so in water).  I am not exaggerating.  I got this
> >info out of one my very favorite materials science books.
> >
> >Don't use Spurr's!
> >
> >
> >Bye,
> >hildy
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 10:01:09 -0500
> From: "Steven E. Slap" <ebs@ebsciences.com>
> Subject: position available
>
> Dear colleagues,
>
> Energy Beam Sciences has a position available for a Sales
> Executive/Product Specialist at our facility in Agawam, Massachusetts.
> Anyone interested in more details should contact me back-channel.
>
> Best regards,
> Steven E. Slap, Vice-President
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 10:16:15 -0500
> From: "Tarpley, John" <jtarpley@amgen.com>
> Subject: RE: Neg Immunocontrols
>
> The point on polyclonal antisera is well taken and that's why, whenever
> possible, we use preimmune serum from the same animal or pool of animals
> used to produce the antibody diluted to the same Ig concentration as the
> primary antibody. By using this control you can see if any "background"
> antibodies already present in the serum will affect your results.
> Unfortunately, this is rarely possible when using commercial antibodies. Can
> anyone suggest a true negative control for polyclonals in this case?
>
> John Tarpley 15-2-B
> Specialist Image Analysis & Immunohistochemistry
> Amgen Inc
> One Amgen Center Drive
> Thousand Oaks, CA  91320
>
> > ----------
> > From:         brian chelack[SMTP:chelack@admin3.usask.ca]
> > Sent:         Wednesday, October 14, 1998 5:07 PM
> > To:   A. Mark Briones
> > Cc:   HistoNet Server
> > Subject:      Re: Neg Immunocontrols
> >
> >
> >
> > Just a quick observation regarding the use of "non-immune sera" as a
> > negative
> > control.  Investigators should be aware that non immune sera will always
> > contain
> > antibodies to other common pathogens that the negative control animal has
> > experienced in its past.  A case in point in our lab was the use of
> > "non-immune
> > rabbit sera as a control alongside a rabbit antisera for Bovine
> > respiratory syncitial
> > virus.
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 10:45:12 -0500
> From: "Montague, Donna C" <MontagueDonnaC@exchange.uams.edu>
> Subject: RE: enclosures
>
> Linda and others:
>
>  In addition to the items you mentioned, I discovered that in Microsot mail
> products when you enter an address into your address book, there is a small
> box you can check (or not) to send items in rich text format. If this box is
> checked the Histonet signals that you have sent an enclosure. Simply
> un-check this box (send messages in plain text format)and you'll stop
> getting the enclosures reply from the Histonet server. Anyway, it worked for
> me.
>
> Donna Montague
> UAMS Orthopaedic Research
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 10:53:58 -0500
> From: "Sarah Ann Christo" <schristo@CVM.TAMU.EDU>
> Subject: Re: Histonet Soliciters -Reply
>
> Dear Elfi,
>    I don't think this is the first time this
> situation has happened regarding your
> company and the Histonet.  I have noticed
> you are fairly aggressive, more than any
> other company, on the Histonet.
>   I feel this way, if a company has a good
> product, it will sell itself by word of mouth.
> I am totally put off by pushy salesmanship.
>   -Sarah at Texas A&M
>
> Dear Mark & Carrie Byrne;
>
> As the head of the head office I am responding to your e-mail, and quite
> frankly I am a bit shocked! I am sorry to hear of your aggressive sentiments
> towards Hacker, and on behalf of the same, I offer personal and corporate
> apologies if you have been in any way offended.
>
> I have checked with our direct sales people, and none of them have any record
> of speaking with you. As you may or may not know, we work with a number of
> independents nationwide and they may or may not have spoken with you
>regarding
> automated coverslipping. Most of us, who are involved with this industry are
> on the HistoNet. It is normal, and I don't think inappropriate, to expect
>some
> type of follow up when questions regarding products and their performance are
> aired over the HistoNet. Vendors do this, because it is our job to know how
> our instruments and our competitors are doing out there. Our customers are
> also one of our best source of ideas which can hopefully be used towards
> improving our products to better meet their needs. This can only happen
> through an open exchange of information.
>
> It would be very helpful, and truly appreciated, if you would provide me with
> the details regarding the incident with a Hacker Rep which you find so
> offensive. Who was the Rep; and what issue did they respond to?  What
>could be
> so reprehensible that it could incite you to vent such an aggressive reaction
> toward an entire company and question our sales practices? Believe me, if
>it's
> that bad, I certainly need to know! I might also add, that it may be more
> effective to contact "The Company", (that would me in this case), directly to
> air your views and vent your anger at someone you could hopefully give you
> some answers, rather than making inflammatory blanket statements over the
> HistoNet, don't you think?
>
> What you should know, is that Hacker has been in this business since 1942. We
> take our business rather seriously. As a company, and as individuals (many of
> whom are histotechs), we make every effort to support this industry, it's
> Societies, affiliates, etc., including the HistoNet.
>
> A rational explanation for your comments would be most welcome. Hopefully
>your
> input can help us avoid this type of situation in the future.
>
> I look forward to hearing from you at your earliest convenience.
>
> Sincerely,
>
> Elfi Hacker
> President
> HACKER Instruments & Industries Inc
>
> Tel. (973) 226-8450
> Fax.(973) 808-8281
> e-mail: HACKERLAB@AOL.COM
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 10:54:26 -0500
> From: "Sarah Ann Christo" <schristo@CVM.TAMU.EDU>
> Subject: Re: email address/vendor -Reply
>
> Dear Gayle,
>   Good advice, there are some that I delete as
> soon as I see the name.  -Sarah
>
> Gayle,
> Amen and Amen!
> Wanda
>
> Gayle Callis wrote:
> >
> > To the lady looking for Anatech email address, they will automatically
> > contact you with your message to me.  Hopefully Dick Dapson or Ada Feldman
> > will see your inquiry to me.
> >
> > As for having vendors contact me through Histonet, I have appreciated
> > their participation and answering MY specific needs and others.  I think
> > they are entitled to being on the list, whether some people like it or
> > not, since this is a way for them to, through our discussions:
> >
> > 1.  Improve their products/provide explanation/clarification on products
> > 2.  Answer peoples' gripes, gritches, etc.
> > 3.  Provide faster service to those asking about a product
> >
> > If I don't like it, I DELETE IT!  This also applies to ANY messaging
> > I am not interested in, vendors or nonvendors!  True, some vendors may
> > be more assertive than others, but that is liveable.  Bashing them
> > is just as aggressive towards them as one perceives they are
> > aggessive towards you, so DELETE IT, relax - life is too short, or
> > you can do what I have done if it is too much - UNSUBCRIBE for a while.
> >
> > Have a good day, the sun is shining in Montana, USA!
> >
> > Gayle Callis
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 11:14:11 -0500
> From: Gayle Callis <uvsgc@msu.oscs.montana.edu>
> Subject: That word again!
>
> After my long "vendor" message, I apologize for misspelling
> that word again --- subscribe or with "un" in front of it!
> I blame it on my fingers!
> Gayle Callis
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 11:30:40 -0500
> From: Cindy Farman <cfarman@sierrabiomedical.com>
> Subject: Test message - please ignore
>
> Test message.
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 11:40:39 -0500
> From: "Richard Roark" <brsales@brinstrument.com>
> Subject: Re: That word again!
>
> Dear Scully,
>
> Do you see a pattern developing here!!!
>
> Mulder
> - -----Original Message-----
> From: Gayle Callis <uvsgc@msu.oscs.montana.edu>
> To: histonet@Pathology.swmed.edu <histonet@Pathology.swmed.edu>
> Date: Thursday, October 15, 1998 9:14 AM
> Subject: That word again!
>
> >After my long "vendor" message, I apologize for misspelling
> >that word again --- subscribe or with "un" in front of it!
> >I blame it on my fingers!
> >Gayle Callis
> >
> >
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 11:50:39 -0500
> From: Decalchem@aol.com
> Subject: Re: Histonet Soliciters (Ominous spam)
>
> In a message dated 10/14/1998 6:38:27 PM, ODDBALSTER@aol.com wrote:
>
> <<leave the soliciting on the "net"!  I would have never been upset had I
> received some email via my name on the list, but SOLICITING ME AT WORK IS
> UNACCEPTABLE! So.......Vendors and so called "Head Hunters" BEWARE! HISTO
> TECHS UNITE!!!!!!!           >>
>
> Once again, vendor bashing has raised its ugly head!
>
> This seems very bizzare and offensive to me.  Our company has contributed
> significant amounts of money to the histonet.  We pay thousands of dollars to
> exhibit at the NSH symposium every year and we are swamped by histotechs
> asking for free handouts such as coffee mugs and teddy bears and samples of
> our products.   I personally get online every morning to field questions that
> might concern my products or my expertise.  We have hundreds of customers,
> each with different experiences.  This knowledge is valuable to
>histotechs who
> are experiencing difficulties.     I offer advice, samples of products,
> methodologies,  copies of techinical articles, all free of charge.
>
> Perhaps you would rather stick your head in the sand and buy your laboratory
> necessities from one single source without regard to price, service or
> quality.  Perhaps your lab runs without problems and you never need to speak
> with a company rep.  That's good for you.  My job is to manufacture and
>market
> the best products I can.  Telemarketing is a large part of our sales
>strategy.
> We gather phone numbers and adresses from whatever sources are available.  If
> we call you, and you don't wish to speak with us just say so.   However,
>there
> is no need to get all worked up and nasty about it.
>
> By the way, call me anytime you like if you have a problem. I'll even pay for
> the call.
>
> Cliff Berger
> President
> Decal Chemical Corp
> 1-800-428-5856
> email:decalchem@aol.com
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 12:00:43 -0500
> From: Cindy Farman <cfarman@sierrabiomedical.com>
> Subject: RE: enclosures
>
> Hi everyone,
>
> I was having the enclosure problem so I had one of our computer guys try to
> fix it. He did something that was supposed to stop enclosures, but it
> didn't work (I don't know what he did). So I tried Donna's suggestion
> below, and it worked. I did have the rich text format box checked and I
> unchecked it and now I can send messages to histonet!
>
> Thanks Donna,
>
> Cindy Farman
>
> - -----Original Message-----
> From:   Montague, Donna C [SMTP:MontagueDonnaC@exchange.uams.edu]
> Sent:   Thursday, October 15, 1998 8:31 AM
> To:     'LINDA MARGRAF MD'; Bright@dial.pipex.com;
>HistoNet@Pathology.swmed.edu
> Subject:        RE: enclosures
>
> Linda and others:
>
>  In addition to the items you mentioned, I discovered that in Microsot mail
> products when you enter an address into your address book, there is a small
> box you can check (or not) to send items in rich text format. If this box
> is
> checked the Histonet signals that you have sent an enclosure. Simply
> un-check this box (send messages in plain text format)and you'll stop
> getting the enclosures reply from the Histonet server. Anyway, it worked
> for
> me.
>
> Donna Montague
> UAMS Orthopaedic Research
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 13:30:29 -0500
> From: "Barry Rittman" <brittman@mail.db.uth.tmc.edu>
> Subject:
>
> Might I suggest that we discontinue the discussion re vendors?
> Most vendors have, and will continue to use their common sense in their
> interactions with the Histonet.
> Let us get back to the business that this Histonet is famous for, the
> dissemination of knowledge and the availability of expertise in several
> fields.
> thank you.
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 13:40:12 -0500
> From: VICKI_GAUCH@CCGATEWAY.AMC.EDU
> Subject: Histonet Solicitors
>
>      I have to agree that having some salesperson get your name from the
>      Histonet and then using that to solicit business is very annoying.  I
>      would not mind receiving an E-mail message (if I had to be contacted
>      at all) stating what it is that they want from me but I do not
>      appreciate  unsolicited sales calls in the lab.  I have had this
>      experience many times and a simple "NO" as was suggested was not well
>      accepted by the sales person and resulted in several more annoying
>      calls to follow.     I DO realize that many companies make donations
>      of money,time and samples for our conventions and am very grateful for
>      their kindness  and I do appreciate their answers to our questions. I
>      just think a little consideration for our technical time at work would
>      be nice.  If I received an E-mail from someone who saw my name on the
>      Histonet and it was something I was interested in looking at I would
>      be more than happy to set up an appointment to speak with that
>      person.....Just don't call me in the middle of grossing,etc. UGH !!!
>
>      Vicki Gauch
>      Albany Medical Center
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 14:45:59 -0500
> From: "Ford M. Royer" <froyer@bitstream.net>
> Subject: Re:Vendors
>
> Alluding to the analogy that I believe Russ Allison made a while back,
> ...the dog has now run through the flock of seagulls and returned home.  It
> is time for the birds to settle back down on the beach and go about their
> business, while the tide ebbs and flows peacefully until it is time, once
> again, for the dog to be let out to run along the shore.    :)
>
> Barry Rittman wrote:
>
> > Might I suggest that we discontinue the discussion re vendors?
> > Most vendors have, and will continue to use their common sense in their
> > interactions with the Histonet.
> > Let us get back to the business that this Histonet is famous for, the
> > dissemination of knowledge and the availability of expertise in several
> > fields.
> > thank you.
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 14:55:30 -0500
> From: ODDBALSTER@aol.com
> Subject: Re: Histonet Solicitors
>
> amen Vicki :o)
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 15:06:38 -0500
> From: VICKI_GAUCH@CCGATEWAY.AMC.EDU
> Subject: Re[2]: email address/vendor -Reply
>
>      Gayle,
>        I don't mind vendors responding on the Histonet either.  As long as
>      they are using it to respond to technical questions or inquire about
>      something I'm no bothered by it.  What I don't like is the sales phone
>      calls that can result from our names being seen on the
>      Histonet...whole other story....
>
>      Vicki
>
> ______________________________ Reply Separator
> _________________________________
> Subject: Re: email address/vendor -Reply
> Author:  Sarah Ann Christo <schristo@CVM.TAMU.EDU> at Internet-Mail
> Date:    10/15/98 12:37 PM
>
> Dear Gayle,
>   Good advice, there are some that I delete as
> soon as I see the name.  -Sarah
>
>
> Gayle,=20
> Amen and Amen!
> Wanda
>
> Gayle Callis wrote:
> >=20
> > To the lady looking for Anatech email address, they will automatically
> > contact you with your message to me.  Hopefully Dick Dapson or Ada =
> Feldman
> > will see your inquiry to me.
> >=20
> > As for having vendors contact me through Histonet, I have appreciated
> > their participation and answering MY specific needs and others.  I think
> > they are entitled to being on the list, whether some people like it or
> > not, since this is a way for them to, through our discussions:
> >=20
> > 1.  Improve their products/provide explanation/clarification on products
> > 2.  Answer peoples' gripes, gritches, etc.
> > 3.  Provide faster service to those asking about a product
> >=20
> > If I don't like it, I DELETE IT!  This also applies to ANY messaging
> > I am not interested in, vendors or nonvendors!  True, some vendors may
> > be more assertive than others, but that is liveable.  Bashing them
> > is just as aggressive towards them as one perceives they are
> > aggessive towards you, so DELETE IT, relax - life is too short, or
> > you can do what I have done if it is too much - UNSUBCRIBE for a while.
> >=20
> > Have a good day, the sun is shining in Montana, USA!
> >=20
> > Gayle Callis
>
>
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 15:07:09 -0500
> From: <general@silcom.com>
> Subject: RE: Apoptotic Markers for Rat Tissue
>
> Re: Apoptosis
>
> >Another possibility you might look into is the LeY antigen. If I remember
>
> correctly, I think it recognizes a carbohydrate antigen in mouse, so it
> may work in rat tissue. I think DAKO sells this antibody; but, I am not
> surprised that a DAKO rep has not answered<
>
> DAKO Code Number = M4504
>
>  I don't have any cross reactivity info for Lewis Y Mouse Monoclonal
> (Clone BM-1, Isotype IgM, kappa), but if you contact DAKO Technical
> Services (800-424-0021), they might be able to help you.
>
> Bret Cook
> DAKO Corp.
>
> - ----------
> From:   tylee[SMTP:tylee@itis.com]
> Sent:   Wednesday, October 14, 1998 7:44 PM
> To:     histonet@Pathology.swmed.edu
> Subject:        Re: Apoptotic Markers for Rat Tissue
>
> Christina,
>
> Apoptosis is sort of a hobby of mine.  I do not think you should rely on
> the p53 or Bax antibodies you mentioned as markers of apoptosis.  To my
> knowledge, there are no good antibodies to detect markers of apoptosis for
> rat tissue (that are commercially available). There are some that are
> patent pending in pharmaceutical companies that are not generally available
> even to academic researchers.
> There are some other antibodies that you might investigate further
> including the Apo 2.7 antibody developed by Schlossman (sp?) at Harvard and
> sold by Immunotech (or Beckman/Coulter or whatever that company is called
> now).  It recognizes an unidentified mitochondrial surface protein; but I
> am not sure if there is any evidence that it works in rat.
> Another possibility you might look into is the LeY antigen. If I remember
> correctly, I think it recognizes a carbohydrate antigen in mouse, so it may
> work in rat tissue. I think DAKO sells this antibody; but, I am not
> surprised that a DAKO rep has not answered. Given the recent bashing of
> Hacker and other solicitors, I suspect all the companies are a little
> gun-shy.
> I agree with Russ Allison that morphology may be the most reliable if you
> know what to look for and TUNEL is a good alternative to detect the late
> stages of apoptosis; however, proper controls are necessary because of the
> possibility of artifacts. There are several variations of the "TUNEL"
> reaction that you should look into if you choose to label DNA fragments.
> If you are just getting started in detecting apoptosis, my recommendation
> would be TUNEL.
>
> Ty Lee
> tylee@itis.com
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 15:45:17 -0500
> From: "LEROY BROWN" <lhbhcs@pioneernet.net>
> Subject: Re: Re:Vendors
>
> AMEN
> - -----Original Message-----
> From: Ford M. Royer <froyer@bitstream.net>
> To: Histonet@Pathology.swmed.edu <Histonet@Pathology.swmed.edu>
> Date: Thursday, October 15, 1998 12:50 PM
> Subject: Re:Vendors
>
> >Alluding to the analogy that I believe Russ Allison made a while back,
> >...the dog has now run through the flock of seagulls and returned home.  It
> >is time for the birds to settle back down on the beach and go about their
> >business, while the tide ebbs and flows peacefully until it is time, once
> >again, for the dog to be let out to run along the shore.    :)
> >
> >Barry Rittman wrote:
> >
> >> Might I suggest that we discontinue the discussion re vendors?
> >> Most vendors have, and will continue to use their common sense in their
> >> interactions with the Histonet.
> >> Let us get back to the business that this Histonet is famous for, the
> >> dissemination of knowledge and the availability of expertise in several
> >> fields.
> >> thank you.
> >
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 15:55:38 -0500
> From: ODDBALSTER@aol.com
> Subject: Re: Vendors....last email from me, delete if uninterested! I promise
> :o)
>
> Mr.Berger,
>                   Obviously you have misunderstood my intentions and/or
> communication was not clear here! I never "bashed" you or your company for
> offering your expertise or your products on the Histonet or any other
> listserver for that matter, I simply do not feel it is "professional
>courtesy"
> to call one at work when one did not offer even a phone number and/or even a
> product RELATED comment. I personally have not been contacted from a
>vendor, I
> was however contacted by a "headhunter", hence, my email. The vendor mention
> was in reference to another email I received regarding another tech who was
> solicited by a company "pushing" their product and didn't feel it was
> acceptable, I apologize if I was unclear.
>                     I am sure your company has contributed significant
>amounts
> of money
> to these organizations, no need to strike up a defense on my account! However
> isn't that a part of advertising, much like your "sales strategy"? But
>you are
> correct, unfortunately my laboratory has a vendor contract, much to my
> repugnance, and I do not have much choice in the way of products. If things
> were different, I might even give your company a try, you seem to defend it
> with pride.......
>
> **"Perhaps you would rather stick your head in the sand and buy your
> laboratory necessities from one single source without regard to price,
>service
> or quality.  Perhaps your lab runs without problems and you never need to
> speak with a company rep.  That's good for you. However, there is no need to
> get all worked up and nasty about it.  By the way, call me anytime you
>like if
> you have a problem. I'll even pay for the call."**
>                      Now WHO is getting all worked up and nasty?? Good
>sarcasm
> is always appreciated here!  :o)
>
>                       Let's get back to technical questions and answers, what
> this list was intended for.......I apologize for the lengthy debate in
>which I
> started!! (Please don't damn me!!)  :o)
>
> K.M.Z. HT,MLT
> Lead Histo Tech
> Somewhere,FL
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 16:46:43 -0500
> From: "Sarah Ann Christo" <schristo@CVM.TAMU.EDU>
> Subject: Histonet/prowrestling
>
> Histonetters,
> I use to think the Histonet was like driving
> on the freeway.  This week it's been
> Professional Wrestling.  I especially liked the
> body slams and head locks.
>   Have a good weekend,  Sarah, Texas A&M
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 16:56:13 -0500
> From: "Sarah Ann Christo" <schristo@CVM.TAMU.EDU>
> Subject: Re:Vendors -Reply
>
> Dear Ford,
>   That was great, just one thing-who is the
> dog and who are the seagulls???  -Sarah
>
> Ford wrote:
> Alluding to the analogy that I believe Russ Allison made a while back,
> ...the dog has now run through the flock of seagulls and returned home.  It
> is time for the birds to settle back down on the beach and go about their
> business, while the tide ebbs and flows peacefully until it is time, once
> again, for the dog to be let out to run along the shore.    :)
>
> Barry Rittman wrote:
>
> > Might I suggest that we discontinue the discussion re vendors?
> > Most vendors have, and will continue to use their common sense in their
> > interactions with the Histonet.
> > Let us get back to the business that this Histonet is famous for, the
> > dissemination of knowledge and the availability of expertise in several
> > fields.
> > thank you.
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 18:01:11 -0500
> From: sjrugby@juno.com (SANDY J JULSING)
> Subject: Re: Histonet/prowrestling
>
> Sarah,
>         How dare you tittilate me with a subject line that has the words
> Histonet AND pro-wrestling and than have nothing in the message area?  Is
> this some kind of cruel joke?
>                                 Sandy - Steroid Free - Julsing
>
>
> On Thu, 15 Oct 1998 16:46:28 -0500 Sarah Ann Christo
> <schristo@CVM.TAMU.EDU> writes:
> ><<< No Message Collected >>>
> >
>
> ___________________________________________________________________
> You don't need to buy Internet access to use free Internet e-mail.
> Get completely free e-mail from Juno at http://www.juno.com
> or call Juno at (800) 654-JUNO [654-5866]
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 19:00:19 -0500
> From: bamur@alaska.net
> Subject: Re: histonet solicitors poll
>
> Ms Louise Taylor wrote:
> >
> > Well, here's my 2 cents worth,
> >
> > If decisions on equipment purchases etc are not your decision then
> > you should not be getting steamed up about vendors contacting you,
> > you should just politely tell them that if necessary they should
> > contact your supervisor.
> > I feel that people using the histonet as a market survey tool is just
> > peachy - at least they know what competition is out there and whether
> > their sales  tactic and product is suitably market oriented. I have
> > often suggested to vendors other possible targets for
> > their product - people that i know are looking for a particular item
> > or have an interest in a particualr field - I do not consider this
> > inappropriate - I hope that other s will do the same for me.
> >
> > Life is too short to get agitated on such issues
> >
> > Louise taylor
> >
> > On 14 Oct 98 at 20:57, Mark & Carrie Byrne wrote:
> >
> > > hi all,
> > > i'd like to get others opinions about the current debate about this.
> > > here's my feeling:
> > > i've no problem at all with sales and tech reps giving info or advice on
> the
> > > topics that come up, just like any other histonet subscriber (hey....i
> > > spelled it right!!  there's hope for me yet :-)).  however, i'd rather
> that
> > > the line be drawn at that.  personally, i do not welcome sales pitches
> from
> > > out of the blue.  especially since purchasing decisions are the
> > > supervisor's, not mine.
> > >
> >
> > > ok, end of diatribe from me.
> > >
> > > carrie kyle-byrne
> > > eire@teleport.com
> > >
> > >
> > >
>
> Well said Louise!! Let's smile at these little things and other things
> that may annoy us!
> Have a great day and a good weekend!
>
> B.A. Murray
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 19:30:16 -0500
> From: sehansen@erols.com
> Subject: Re:
>
> Amen!
>       When not happy with an e-mail or recognize an e-mailer as one
> that you for some reason do not wish to hear their opinion - Hit the
> DELETE button.  There have been several discussions that I have
> disagreed with and some "netters" who's opinion I have been diametically
> (sp) oposed to and I still read then delete.  ALL persons who are
> engaged in Histology are welcome here,  we need to be tolerent and use
> the delete when needed.
>    Stan
>
> Barry Rittman wrote:
> >
> > Might I suggest that we discontinue the discussion re vendors?
> > Most vendors have, and will continue to use their common sense in their
> > interactions with the Histonet.
> > Let us get back to the business that this Histonet is famous for, the
> > dissemination of knowledge and the availability of expertise in several
> > fields.
> > thank you.
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 20:00:17 -0500
> From: Nora Fox <nefox@leland.Stanford.EDU>
> Subject: microscope bulbs
>
> Hello!!!  I have looked in all of my catalogs & cannot find a source for a
> microscope bulb.  The scope is an old American Optical.  Can anyone help me?
>
> Thank you all for your help.
>
> Nora
> Nora Fox
> Division of Orthopaedic Surgery
> Stanford University
> Edwards R144, MC 5341
> 300 Pasteur Drive
> Stanford, CA 94305
> phone: (650)725-2746
> fax:  (650)723-6396
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 20:30:20 -0500
> From: al.floyd@juno.com (Alton D. Floyd)
> Subject: Microscope Bulbs
>
> Nora,
> I have had great success in finding bulbs for very old microscopes and
> illuminators from Bulbs Direct, 800-772-5267.  They also have a web site,
> www.bulbdirect.com
>
> For the record, I have no interest in this company - I am just a
> satisfied customer.
> Al Floyd
>
> ___________________________________________________________________
> You don't need to buy Internet access to use free Internet e-mail.
> Get completely free e-mail from Juno at http://www.juno.com
> or call Juno at (800) 654-JUNO [654-5866]
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 20:39:11 -0500
> From: DayDawning@aol.com
> Subject: Re: Vendors -Reply
>
> In a message dated 10/15/98 6:13:09 PM Eastern Daylight Time,
> schristo@CVM.TAMU.EDU writes:
>
> << ear Ford,
>    That was great, just one thing-who is the
>  dog and who are the seagulls???  -Sarah
>
>  Ford wrote:
>  Alluding to the analogy that I believe Russ Allison made a while back,
>  ...the dog has now run through the flock of seagulls and returned home.  It
>  is time for the birds to settle back down on the beach and go about their
>  business, while the tide ebbs and flows peacefully until it is time, once
>  again, for the dog to be let out to run along the shore.    :)
>   >>
> I don't know about you other vendors but I'm feeling a little like the
>seagull
> droppings.......
> Dawn Truscott
>
> ----------------------------------------------------------------------
>
> Date: 15 Oct 1998 21:00:18 -0500
> From: "P. Emry" <emry@u.washington.edu>
> Subject: Re: Histonet/prowrestling
>
> HistoMania, Histoman...Histotech turned pro-wrestler!
> His costume would be an old lab coat with purple and orange stain spots on
> it.  Maybe a cape of the same material, or better yet, congo red.
> He could carry a see-through container with identifiable parts, grey from
> formaldehyde, along with forceps and slides in the other hand.
>
> Lord, wish I knew how to send this anonymously.
>
> On Thu, 15 Oct 1998, SANDY J JULSING wrote:
>
> > Sarah,
> >       How dare you tittilate me with a subject line that has the words
> > Histonet AND pro-wrestling and than have nothing in the message area?  Is
> > this some kind of cruel joke?
> >                               Sandy - Steroid Free - Julsing
> >
> >
> > On Thu, 15 Oct 1998 16:46:28 -0500 Sarah Ann Christo
> > <schristo@CVM.TAMU.EDU> writes:
> > ><<< No Message Collected >>>
> > >
> >
> > ___________________________________________________________________
> > You don't need to buy Internet access to use free Internet e-mail.
> > Get completely free e-mail from Juno at http://www.juno.com
> > or call Juno at (800) 654-JUNO [654-5866]
> >
> >
>
> Here are the messages received yesterday!



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