Re: Staining problem details
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From: | Michelle McCafferty <mmccaff@mbl.edu> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Hello Pam,
Since you are using an alcoholic eosin try dipping the basket back
into either 95 or 80% dehydrant before going into the Eosin stain. I use
the last 95% in the deparaffinizing series for approximately 30 seconds,
then on into the Eosin with a couple of dips for dispersing the stain
evenly over the slide surface. Never any patchy staining problems.
Hope this helps!
Michelle
Michelle McCafferty
Marine Biological Laboratory
7 MBL Street
Woods Hole, MA 02543-1015
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