Re: Staining problem details

<< Previous Message | Next Message >>
From:Michelle McCafferty <> (by way of histonet)
To:histonet <>
Content-Type:text/plain; charset="us-ascii"

Hello Pam,

	Since you are using an alcoholic eosin try dipping the basket back
into either 95 or 80% dehydrant before going into the Eosin stain.  I use
the last 95% in the deparaffinizing series for approximately 30 seconds,
then on into the Eosin with a couple of dips for dispersing the stain
evenly over the slide surface.  Never any patchy staining problems.

	Hope this helps!

Michelle McCafferty
Marine Biological Laboratory
7 MBL Street
Woods Hole, MA 02543-1015

<< Previous Message | Next Message >>