Re: Staining problem details

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From:Michelle McCafferty <mmccaff@mbl.edu> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Hello Pam,

	Since you are using an alcoholic eosin try dipping the basket back
into either 95 or 80% dehydrant before going into the Eosin stain.  I use
the last 95% in the deparaffinizing series for approximately 30 seconds,
then on into the Eosin with a couple of dips for dispersing the stain
evenly over the slide surface.  Never any patchy staining problems.

	Hope this helps!
	Michelle


Michelle McCafferty
Marine Biological Laboratory
7 MBL Street
Woods Hole, MA 02543-1015




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