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From:Steve Traer <> (by way of histonet)
To:histonet <>
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As far as I am aware the only way to properly disinfect a cryostat is
to completely defrost it,  place a container of formaldehyde in it
overnight with the lid closed and then replace with  a container of
ammonia for 30 minutes the next morning.  Then,wearing gloves,
thoroughly wipe out the chamber with meths.  If you have to remove the
microtome wash it in meths and reassemble using the appropriate oil.

We always wear gloves when cutting frozen sections and when hand
mounting, some also wear gloves when embedding.

I have not used an open flame for embedding since 1981, like you we
find that three pairs of forceps in the wells of our Leica
Histoembedder are sufficient although many people in the UK use heated

Steve Traer
Queen Alexandra Hospital

---SANDY J JULSING  wrote:
> Histology comrades,
> 	I am "the new guy" to the lab and am concerned about the
> following possible safety issues...
> -Open flame. (bunsen burner)  I personally haven't used an open
flame for
> embedding since       1984...  If find several forceps in the heated
> wells quite adequate.  We've already had one          flaming
garbage can
> (that was hidden from the lab manager) and singed log sheets...
> -Glove-less frozen sections - Currently one other tech (also new) and
> myself are the only ones    to use gloves while cutting a frozen
>  The other 5 techs use their bare-naked hands.
> -Glove-less coverslipping - Again the new tech and myself are the only
> ones to use gloves.
> Don't want to be a "tattle-tale" to the lab manager and I have
brought it
> up to the "Team", but no one seems concerned except me.  Any
> comments/suggestions?
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