Re: Quenching peroxidase activity??!!??
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From: | Barbara D Wright <bwright@gene.COM> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
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Content-Type: | text/plain; charset="us-ascii" |
Histonetters;
I agree with carrie Kyle-byrne - I too use the glucose/glucose oxidase
method of endogenous peroxidase on my frozen sections of rodent (murine
and rat) tissues with great success and no disruption of morphology. On
my paraffin sections I use a peroxidase block from
Kirkegaard & Perry with is a periodate based solution for their
recommended time of 4 minutes.
Both of these eproxidase blockers give good results with no disruption
of morphology.
Barb Wright :)
Genentech, Inc.
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