Re: Quenching peroxidase activity??!!??

<< Previous Message | Next Message >>
From:Barbara D Wright <bwright@gene.COM> (by way of histonet)
To:histonet <>
Content-Type:text/plain; charset="us-ascii"


I agree with carrie Kyle-byrne  - I too use the glucose/glucose oxidase
method of endogenous peroxidase on my frozen sections of rodent (murine
and rat) tissues with great success and no disruption of morphology.  On
my paraffin sections I use a peroxidase block from
Kirkegaard & Perry with is a periodate based solution for their
recommended time of 4 minutes.
Both of these eproxidase blockers give good results with no disruption
of morphology.

Barb Wright :)
Genentech, Inc.

<< Previous Message | Next Message >>