Re: LDH (lactate dehydrogenase) stain
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
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On Thu, 5 Nov 1998, Pam Plumlee wrote:
> Histonetters: Has anyone out there heard of or done a Lactate
> Dehydrogenase stain on formalin fixed or frozen human tissue? My
> researcher has asked if I can do this stain to demonstrate thermal
> damage in skin. Any leads would be greatly appreciated.
> pplumlee@thermolase.com
Unlike most dehydrogenases, lactate dehydrogenase activity
survives moderate formaldehyde fixation, so you don't have
to work with unfixed cryostat sections. There are cytoplasmic
and mitochondrial forms of the enzyme. Histochemical
localization is done with a tetrazolium salt, and NAD+ as
the coenzyme.
Biochemists often assay the LDH that oozes out of bits
of tissue, to get a measurement of cellular damage following
various experiments. If a cell has been wrecked, the
enzyme leaks out into the extracellular fluid. I don't know
if this could be seen histochemically or not.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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