Is this what you were lookin for?
"...We know no reason why gunpowder treason
should ever be forgot."    From the book "1066 and all That", WC Sellar and
Yeatman, 1930.   (So, I'm a book-nerd -- I can't help it!)

Minde Harless
TMH Dept. of Pathology
Craig, CO

----------
> From: HistoNet Server <HistoNet@Pathology.swmed.edu>
> To: HistoNet Server <HistoNet@pathology.swmed.edu>
> Subject: Daily Digest
> Date: Thursday, November 05, 1998 11:04 PM
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 03:00:41 -0600
> From: Cristiano Rumio <cristiano.rumio@unimi.it>
> Subject: Trichohyalin Ab
>
> Dear Friends,
>
> 	I am looking for human trichohyalin antibodies but I am not able to
>find
> any company that ahs this products in its list. Have some of you an idea
> where I can buy them.
>
> Thank in advance
>
> Cristiano
>
>
> _________________________________
>
> Dr. Cristiano Rumio
> Institute of Human Anatomy
> Via Mangiagalli, 31
> 20133 Milan.
> Italy
> Tel. -39-2-2663683
> Fax. -39-2-2364082
> e.mail: cristiano.rumio@unimi.it
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 03:01:05 -0600
> From: ALLISON@cardiff.ac.uk
> Subject: Halloween & Guy Fawkes
>
> O.K., all you guys in the States make a big ball out of halloween -
> of pagan origin, if I remember aright.
>
> Well, to day is OUR big day.
> We celebrate Guy Fawkes - who FAILED to blow up Parliament - never
> quite understood why we celebrate a failure?  Anyway, ever since, on
> the 5th November, we have our own pagan festival whereby we all go to
> great lengths to show our old hero Guy how he SHOULD have gone about
> it.
> Hence, this evening we will all light bonfires, eat hot-dogs and down
> the occasional amber nectar whist letting off fireworks galore!  The
> sky will be lit up with rockets and sparks, people will be full of
> bonhomie, firemen will earn overtime, casualty departments will be
> full, tomorrow the skin banks will be empty.
>
> Why the hell couldn't Guy Fawkes get it right, then we could
> celebrate Halloween and not ever have to worry about 5 November?
> Russ Allison, Wales
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 03:31:02 -0600
> From: Ian Montgomery <ian.montgomery@bio.gla.ac.uk>
> Subject: Re: Reviews of Cryostats
>
> >Date: Wed, 04 Nov 1998 13:43:49 +0000 (GMT)
> >From: Richard Pitman <r.pitman@zetnet.co.uk>
> >Subject: Re: Reviews of Cryostats
> >To: histonet@pathology.swmed.edu
> >
> >
> >> One of the researchers in our company is in search of a new cryostat.
So
> >> far he has mentioned a Leica,
> >> a Zeiss and a Sakura cryostat (no specific model mentioned).  Are
there
> >> others out there?  Also, he would appreciate any feedback on the
cryostats
> >> from the above mentioned companies; such as the repair record, ease of
use,
> >> ergonomic issues, are you happy with the one you're using, etc.
> >
> >We're happy with our Bright cryostat. Had it for > 12 years, few
> >problems. The Microtome itself is bulletproof, and has needed no
> >attention. A couple of refrigeration problems have been repaired by
> >local refrigeration engineers. Price compares v favourably with
> >similar spec from competitors.
> >
> >Richard, Worcester Royal Infirmary, GB
> >
> 	Have to agree with Richard, but his cryostat is a youngster, mine
> is 25 and still going strong.
> Ian.
>
> Dr. Ian Montgomery,
> West Medical Building,
> University of Glasgow,
> Glasgow,
> G12 8QQ,
> Scotland.
> Tel: 0141 339 8855 Extn. 6602.
> Fax: 0141 330 4100.
> e-mail: ian.montgomery@bio.gla.ac.uk
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 04:30:24 -0600
> From: "Stan Siarkiewicz" <stans@globalnet.co.uk>
> Subject: Re: Halloween & Guy Fawkes
>
> Seems to be a Brit mentality to celebrate failures, maybe it's because we
> don't have enough successes :-(.
> I mean who else would make a hero out of someone who came last in a
sporting
> arena, remember the fuss we made out of Eddie (the Eagle) Edwards.
> Stan
> - -----Original Message-----
> From: RUSS ALLISON <Allison@cardiff.ac.uk>
> To: histonet@pathology.swmed.edu <histonet@pathology.swmed.edu>
> Date: 05 November 1998 09:11
> Subject: Halloween & Guy Fawkes
>
>
> O.K., all you guys in the States make a big ball out of halloween -
> of pagan origin, if I remember aright.
>
> Well, to day is OUR big day.
> We celebrate Guy Fawkes - who FAILED to blow up Parliament - never
> quite understood why we celebrate a failure?  Anyway, ever since, on
> the 5th November, we have our own pagan festival whereby we all go to
> great lengths to show our old hero Guy how he SHOULD have gone about
> it.
> Hence, this evening we will all light bonfires, eat hot-dogs and down
> the occasional amber nectar whist letting off fireworks galore!  The
> sky will be lit up with rockets and sparks, people will be full of
> bonhomie, firemen will earn overtime, casualty departments will be
> full, tomorrow the skin banks will be empty.
>
> Why the hell couldn't Guy Fawkes get it right, then we could
> celebrate Halloween and not ever have to worry about 5 November?
> Russ Allison, Wales
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 05:31:11 -0600
> From: "Saby, Joseph" <Joseph.Saby@wl.com>
> Subject: RE: Sakura VIP Y2K testing
>
> Brings to mind the old adage (I cannot remember to whom to attribute
> it):
>
> 	Any sufficiently advanced technology is indistinguishable from
> magic.
>
> Joe Saby, Parke-Davis, Ann Arbor.
>
> > -----Original Message-----
> > From:	DayDawning@aol.com [SMTP:DayDawning@aol.com]
> > Sent:	Wednesday, November 04, 1998 5:46 PM
> > To:	jmahone@nmhs.org; JC_Suder@fccc.edu;
> > histonet@Pathology.swmed.edu
> > Subject:	Re: Sakura VIP Y2K testing
> >
> > In a message dated 11/4/98 11:20:23 AM Eastern Standard Time,
> > jmahone@nmhs.org
> > writes:
> >
> > <<  Notice how I give human attributes to the equipment.  My limited
> >  understanding of computer technology makes me think that it has to be
> >  either magic or human.
> >  >>
> > So, does the intelligence of the instrument have any bearing on
> > whether you
> > call it a "he" or a "she"?   (  BIG can of worms being opened here.
> > See what
> > happens when I read my e-mail with a glass of wine?)
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 08:15:25 -0600
> From: "Hewlett Bryan  (CMH)" <HEWLETT@EXCHANGE1.CMH.ON.CA>
> Subject: RE: Sakura VIP Y2K testing
>
> Joe,
>
> Attribute it to Arthur C. Clark.
>
> Bryan
>
> >----------
> >From: 	Saby, Joseph[SMTP:Joseph.Saby@wl.com]
> >Sent: 	November 5, 1998 6:18 AM
> >To: 	'DayDawning@aol.com'
> >Cc: 	'HistoNet@pathology.swmed.edu'
> >Subject: 	RE: Sakura VIP Y2K testing
> >
> >Brings to mind the old adage (I cannot remember to whom to attribute
> >it):
> >
> >	Any sufficiently advanced technology is indistinguishable from
> >magic.
> >
> >Joe Saby, Parke-Davis, Ann Arbor.
> >
> >> -----Original Message-----
> >> From:	DayDawning@aol.com [SMTP:DayDawning@aol.com]
> >> Sent:	Wednesday, November 04, 1998 5:46 PM
> >> To:	jmahone@nmhs.org; JC_Suder@fccc.edu;
> >> histonet@Pathology.swmed.edu
> >> Subject:	Re: Sakura VIP Y2K testing
> >>
> >> In a message dated 11/4/98 11:20:23 AM Eastern Standard Time,
> >> jmahone@nmhs.org
> >> writes:
> >>
> >> <<  Notice how I give human attributes to the equipment.  My limited
> >>  understanding of computer technology makes me think that it has to be
> >>  either magic or human.
> >>  >>
> >> So, does the intelligence of the instrument have any bearing on
> >> whether you
> >> call it a "he" or a "she"?   (  BIG can of worms being opened here.
> >> See what
> >> happens when I read my e-mail with a glass of wine?)
> >
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 08:15:53 -0600
> From: rkline@emindustries.com
> Subject: Re: flattening cartilage sections
>
> Alcohol works great with routine, bone and cartilage.  We always kept a
> dish of alcoholic water by the waterbath while cutting.  Never really
> measured how much was put in the dish but I can estimate about 10-15 ml
of
> 95% alcohol:approx. 400 ml of distilled water in a 500 ml staining dish
or
> a dish that was similar.  Some of the technicians preferred lower wider
> rubbermaid type dishes.  It was easier for spreading ribbons.  Just used
a
> dissecting needle or forceps to seperate section in the alcohol bath,
> lifted the section out with a slide, and placed it in the waterbath.   It
> was great for getting wrinkles out of tissue and aided in obtaining nice
> flat sections.  Sections will twirl once placed in the waterbath, but I
> believe this aids in getting the wrinkles out.
>
> Using this method we also did not notice any difference in staining from
> the slides that were floated in the alcohol bath and the slides that were
> not.
>
> Rande Kline HT (ASCP)
> Technical Services
> EM Science
>
>
>
>
> Gayle Callis <uvsgc@msu.oscs.montana.edu> on 11/04/98 04:21:32 PM
>
> To:   histonet@pathology.swmed.edu
> cc:
> Subject:  flattening cartilage sections
>
>
>
>
> I have tried the cold alcohol bath before warm waterbath, but use
> 10% ethanol instead of a higher concentration.  Changed after
> I blew away a few sections.  Have also used a DMSO in 40C waterbath, up
> to 5%, but it requires a glass insert of some type and no contact with
> metal, good ventilation, no skin contact.  Nasty stuff but it worked
> nicely.
>
> Gayle Callis
>
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 09:00:32 -0600
> From: Karen K. Dulany <kkdulany@MAIL.UNMC.EDU>
> Subject: RE: Reviews of Cryostats -Reply
>
> Item Type:  Appointment
> Start Date:  11/05/1998  08:45 am  (Thursday)
> Duration:  1 Hour
>
> To Michelle in Alaska,
> We can definitely sympathize with you about your Sakura cryostat.  We
> purchased a new Sakura cyrostat and only had it a few months or so
> when  it lost power and the refrigerant died.  It had to be sent back
> to California in the big shipping crate and they kept it for two
> weeks.  When it came back again, it only ran for about three days and
> the same thing happened again.  This time the company sent a
> repairman to our lab, and after several tests and putting in new
> parts it still would not run.  After many calls to the company, they
> agreed to send us a replacement, and our pathologist said NO.  So
> they are refunding our complete purchase price and we are going to go
> with another company, not yet decided. We have had many other
> products made by Sakura like the VIP, and our lab-tek embedding
> machines and would highly reccommend them.  There must be some little
> problem with the refrigeration in the cryostats, but I am sure they
> are currently working on the problem and I would not worry about
> buying from them again in the future as I believe they are honest
> people and will resolve your problems also.  Thanks for telling us
> that as we were beginning to wonder if we had electrical problems so
> had our engineers in with all kinds of equipment to check our
> voltage, etc.and found our lab to be OK.
> Karen Dulany HTL (ASCP)
> Marianne Osborne HTL (ASCP)
> Eppley Institute for Cancer Research
> Omaha, NE
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 09:00:58 -0600
> From: "Koutney, Janice" <JKoutney@cytometry.com>
> Subject: Immuno controls for FNA's
>
> Hi All,
> We have just been asked to start doing ER/PR on FNA slides.  My question
> is, what do we use as controls?  A  paraffin block does not seem to be
> an appropriate contol for a fluid.  We do not work in a hospital or a
> histology lab.  We are in a reference lab in which the already embedded
> blocks come to us for immuno staining.  We just cut the slides and
> perform the immunos, so access to FNA fluids or fresh tissues is not an
> option here.  We do have a Shandon Cytospin here.    Any ideas or
> comments will be most appreciated.
>
> Janice Koutny  HT(ASCP)
> Cytometry Asso.
> Brentwood, TN
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 09:21:20 -0600
> From: ayadi@titus.u-strasbg.fr (Abdel)
> Subject: thanks for LacZ staining
>
> Dear Histomembers,
>
> Many thanks for your reply about the "LacZ staining procedure" and the
> "immunostaining procedure after a decalcification step".
>
> Sincerely,
>
> Abdel
>
> Ayadi Abdelkader
> Institut de Genetique et de Biologie Moleculaire et Cellulaire (IGBMC)
> BP 163
> 67404 Illkirch cedex FRANCE
> E-mail: ayadi@igbmc.u-strasbg.fr
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 09:33:08 -0600
> From: "Hoye, Glenda F. (Fka Hood)" <ghoye@iupui.edu>
> Subject: job contacts
>
> One of my students who will be relocating to the Hilton Head area next
fall
> would like a listing of histology lab contacts in that area. She will
> complete this course of study in May, at which time she will be eligible
for
> the ASCP exam; she has prior experience in the lab.
> Contact me personally if you know of labs that she may be able to apply
to
> in the fall of next year.
> Thanks in advance,
> Glenda F. Hoye, BS, HT(ASCP)
> Histotechnology Prog Dir, Indiana Univ Sch of Allied Hlth Sci
> Indianapolis, IN  46202-5119
> 317.278.1599
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 09:33:47 -0600
> From: Karen K. Dulany <kkdulany@MAIL.UNMC.EDU>
> Subject: RE: Reviews of Cryostats -Reply
>
> Item Type:  Appointment
> Start Date:  11/05/1998  09:25 am  (Thursday)
> Duration:  1 Hour
>
> To Michelle in Alaska,
> We can definitely sympathize with you about your Sakura cryostat.  We
> purchased a new Sakura cyrostat and only had it a few months or so
> when  it lost power and the refrigerant died.  It had to be sent back
> to California in the big shipping crate and they kept it for two
> weeks.  When it came back again, it only ran for about three days and
> the same thing happened again.  This time the company sent a
> repairman to our lab, and after several tests and putting in new
> parts it still would not run.  After many calls to the company, they
> agreed to send us a replacement, and our pathologist said NO.  So
> they are refunding our complete purchase price and we are going to go
> with another company, not yet decided. We have had many other
> products made by Sakura like the VIP, and our lab-tek embedding
> machines and would highly reccommend them.  There must be some little
> problem with the refrigeration in the cryostats, but I am sure they
> are currently working on the problem and I would not worry about
> buying from them again in the future as I believe they are honest
> people and will resolve your problems also.  Thanks for telling us
> that as we were beginning to wonder if we had electrical problems so
> had our engineers in with all kinds of equipment to check our
> voltage, etc.and found our lab to be OK.
> Karen Dulany HTL (ASCP)
> Marianne Osborne HTL (ASCP)
> Eppley Institute for Cancer Research
> Omaha, NE
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 09:55:42 -0600
> From: "GS - Cathy Culberson" <cculbers@carolinas.org>
> Subject: re: subscribe digest
>
> subscribe
> cculberson@carolinas.org
> - -------------------------------------
> Name: Cathy Culberson
> E-mail: Cathy Culberson <cculberson@carolinas.org>
> Date: 11/5/98
> Time: 10:47:19 AM
>
> This message was sent by Chameleon
> - -------------------------------------
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 09:56:40 -0600
> From: Pam Plumlee <PPlumlee@thermolase.com>
> Subject: LDH stain
>
> Histonetters:  Has anyone out there heard of or done a Lactate
> Dehydrogenase stain on formalin fixed or frozen human tissue?  My
> researcher has asked if I can do this stain to demonstrate thermal
> damage in skin.  Any leads would be greatly appreciated.
> pplumlee@thermolase.com
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 10:18:26 -0600
> From: Cheryl Crowder <crowder@vt8200.vetmed.lsu.edu>
> Subject: Cryostats
>
> I got into the cryostat discussion late but if you're comparing them,
have
> you tried the Leica.  We have had a manual one for years with only one
> problem - a switch which controlled the object holder.  We could still
cut
> frozens while waiting for its receipt.  There is also a motorized Leica
in
> another department and they love that one.  It is a research department
and
> they need serial and step sections more often than we do.  I haven't seen
> any other messages on the Histonet about the Leica, but we really like
ours.
> Cheryl Crowder, LSU - SVM
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 10:18:50 -0600
> From: Ian Montgomery <ian.montgomery@bio.gla.ac.uk>
> Subject: Zinc formalin.
>
> 	Within the last couple of months there were several postings with
> recipes plus hints and tips for zinc formalin. Unfortunately, before I
> could transfer to my LM formulary (several back-ups and on a server) my
> computer went down the pan. So far down that the hard disc and all its
> contents melted in the Earths core. Please, take me out of my misery and
> post the recipes again.
> Ian.
>
> Dr. Ian Montgomery,
> West Medical Building,
> University of Glasgow,
> Glasgow,
> G12 8QQ,
> Scotland.
> Tel: 0141 339 8855 Extn. 6602.
> Fax: 0141 330 4100.
> e-mail: ian.montgomery@bio.gla.ac.uk
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 11:00:26 -0600
> From: "Gamble,Marilyn S" <Marilyn.S.Gamble@kp.ORG>
> Subject: RE: Halloween & Guy Fawkes
>
> Don't you burn Guy in effigy annually, as part of your bonfire?  (Just to
> punish him every year for his attempt?)
>
> > ----------
> > From: 	RUSS ALLISON[SMTP:Allison@cardiff.ac.uk]
> > Sent: 	Thursday, November 05, 1998 12:50 AM
> > To: 	histonet@Pathology.swmed.edu
> > Subject: 	Halloween & Guy Fawkes
> >
> > O.K., all you guys in the States make a big ball out of halloween -
> > of pagan origin, if I remember aright.
> >
> > Well, to day is OUR big day.
> > We celebrate Guy Fawkes - who FAILED to blow up Parliament - never
> > quite understood why we celebrate a failure?  Anyway, ever since, on
> > the 5th November, we have our own pagan festival whereby we all go to
> > great lengths to show our old hero Guy how he SHOULD have gone about
> > it.
> > Hence, this evening we will all light bonfires, eat hot-dogs and down
> > the occasional amber nectar whist letting off fireworks galore!  The
> > sky will be lit up with rockets and sparks, people will be full of
> > bonhomie, firemen will earn overtime, casualty departments will be
> > full, tomorrow the skin banks will be empty.
> >
> > Why the hell couldn't Guy Fawkes get it right, then we could
> > celebrate Halloween and not ever have to worry about 5 November?
> > Russ Allison, Wales
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 11:12:46 -0600
> From: Pam Plumlee <PPlumlee@thermolase.com>
> Subject: FW: LDH stain
>
>
>
> > -----Original Message-----
> > From:	Pam Plumlee [SMTP:PPlumlee@thermolase.com]
> > Sent:	Thursday, November 05, 1998 7:50 AM
> > To:	'histonet@pathology.swmed.edu'
> > Subject:	LDH stain
> >
> > Histonetters:  Has anyone out there heard of or done a Lactate
> > Dehydrogenase stain on formalin fixed or frozen human tissue?  My
> > researcher has asked if I can do this stain to demonstrate thermal
> > damage in skin.  Any leads would be greatly appreciated.
> > pplumlee@thermolase.com
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 11:13:42 -0600
> From: Corazon Bucana <bucana@audumla.mdacc.tmc.edu>
> Subject: anti-EBA
>
> I would appreciate knowing the full address or phone number of
Sternberger
> Monoclonals, Baltimore, MD.  A paper referenced this company as a source
of
> antibody to a barrier protein.
>
> Thanks,
>
> Cora Bucana
> *******************************************************
> Corazon D. Bucana, Ph.D.
> Department of Cell Biology
> U.T. M.D. Anderson Cancer Center
> 1515 Holcombe Blvd.  Box 173
> Houston, Texas 77030
> Phone: (713) 792-8106
> Email:bucana@audumla.mdacc.tmc.edu
> FAX: (713) 792-8747
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 11:33:30 -0600
> From: Cindy Farman <cfarman@sierrabiomedical.com>
> Subject: RE: Halloween & Guy Fawkes
>
> It's my little girl's 3rd birthday today. When she was born my British
> friend Rachel informed me she was born on Guy Fawke's day. I said "what
the
> heck is that" and she proceeded to inform me about this crazy guy and how

> they celebrate in England by lighting fires and burning effigies of Guy
> Fawkes. I said the Brits would find any excuse to get drunk! At least
> Rachel will never forget Christina's birthday!
>
> Cindy Farman
> - -----Original Message-----
> From:	Stan Siarkiewicz [SMTP:stans@globalnet.co.uk]
> Sent:	Thursday, November 05, 1998 1:51 AM
> To:	RUSS ALLISON; histonet@Pathology.swmed.edu
> Subject:	Re: Halloween & Guy Fawkes
>
> Seems to be a Brit mentality to celebrate failures, maybe it's because we
> don't have enough successes :-(.
> I mean who else would make a hero out of someone who came last in a
> sporting
> arena, remember the fuss we made out of Eddie (the Eagle) Edwards.
> Stan
> - -----Original Message-----
> From: RUSS ALLISON <Allison@cardiff.ac.uk>
> To: histonet@pathology.swmed.edu <histonet@pathology.swmed.edu>
> Date: 05 November 1998 09:11
> Subject: Halloween & Guy Fawkes
>
>
> O.K., all you guys in the States make a big ball out of halloween -
> of pagan origin, if I remember aright.
>
> Well, to day is OUR big day.
> We celebrate Guy Fawkes - who FAILED to blow up Parliament - never
> quite understood why we celebrate a failure?  Anyway, ever since, on
> the 5th November, we have our own pagan festival whereby we all go to
> great lengths to show our old hero Guy how he SHOULD have gone about
> it.
> Hence, this evening we will all light bonfires, eat hot-dogs and down
> the occasional amber nectar whist letting off fireworks galore!  The
> sky will be lit up with rockets and sparks, people will be full of
> bonhomie, firemen will earn overtime, casualty departments will be
> full, tomorrow the skin banks will be empty.
>
> Why the hell couldn't Guy Fawkes get it right, then we could
> celebrate Halloween and not ever have to worry about 5 November?
> Russ Allison, Wales
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 11:45:13 -0600
> From: "vector@vectorlabs.com" <vector@vectorlabs.com>
> Subject: Re: casein information needed
>
> For those who may be interested, Vector Laboratories offers a 10x casein
> solution which dilutes up to 2.5 liters. The catalog number is SP-5020.
> Please let us know if you would like to receive a product specification
> sheet by fax.
>
> Sincerely,
>
>
> Technical Services
>
> Vector Laboratories
> 30 Ingold Road
> Burlingame, CA 94010
>
> Tel:  (650) 697-3600
> Fax:  (650) 697-0339
> E-mail:  vector@vectorlabs.com
> Website:  http://www.vectorlabs.com
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 11:55:44 -0600
> From: marilyn.johnson@agric.gov.ab.ca
> Subject: Chlamydia
>
> Hi Histonetters,
> Thanks to all that replied to my request about Chlamydia.
> For some reason I cannot get E-mail to the 2 following submitters.
> For Michael Manzer - please FAX the Castaneda procedure
>         Kathy Oprea - please  FAX the Gimenez procedure
> Thanks again everyone !!
>
> Marilyn Johnson
> Animal Health Labs.
> Edmonton, Alberta, Canada
>
> FAX: (403) 427-1437
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 12:06:30 -0600
> From: "Kathy Oprea" <koprea@calc.vet.uga.edu>
> Subject: Thank you!
>
> Thank you histonetters and vendors for replying to the casein
> questions. Ya'll are great!
> Kathy
> ************ Thank you ************
> * Kathy Oprea                     *
> * University of Georgia           *
> * College of Veterinary Medicine  *
> * Athens, GA 30602-7388           *
> * KOPREA@calc.vet.uga.edu         *
> The worker who's never said "Thank
> God it's Friday," probably never
> really worked.
> ************************************
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 12:07:14 -0600
> From: Pam Plumlee <PPlumlee@thermolase.com>
> Subject: FW: LDH stain
>
>
>
> > -----Original Message-----
> > From:	Pam Plumlee [SMTP:PPlumlee@thermolase.com]
> > Sent:	Thursday, November 05, 1998 7:50 AM
> > To:	'histonet@pathology.swmed.edu'
> > Subject:	LDH stain
> >
> > Histonetters:  Has anyone out there heard of or done a Lactate
> > Dehydrogenase stain on formalin fixed or frozen human tissue?  My
> > researcher has asked if I can do this stain to demonstrate thermal
> > damage in skin.  Any leads would be greatly appreciated.
> > pplumlee@thermolase.com
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 12:26:32 -0600
> From: Pam Plumlee <PPlumlee@thermolase.com>
> Subject: FW: LDH stain
>
>
>
> > -----Original Message-----
> > From:	Pam Plumlee [SMTP:PPlumlee@thermolase.com]
> > Sent:	Thursday, November 05, 1998 8:59 AM
> > To:	'HistoNet@Pathology.swmed.edu'
> > Subject:	FW: LDH stain
> >
> >
> >
> > > -----Original Message-----
> > > From:	Pam Plumlee [SMTP:PPlumlee@thermolase.com]
> > > Sent:	Thursday, November 05, 1998 7:50 AM
> > > To:	'histonet@pathology.swmed.edu'
> > > Subject:	LDH stain
> > >
> > > Histonetters:  Has anyone out there heard of or done a Lactate
> > > Dehydrogenase stain on formalin fixed or frozen human tissue?  My
> > > researcher has asked if I can do this stain to demonstrate thermal
> > > damage in skin.  Any leads would be greatly appreciated.
> > > pplumlee@thermolase.com
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 12:28:00 -0600
> From: Heike Grabsch <h.grabsch@uni-koeln.de>
> Subject: 17-1-A- antigen
>
> Hi folks,
>
> I trying to locate a source for an antibody which works in paraffin
> sections and recognizes the 17-1 A antigen.
>
> I hope someone of you can give me hint.
>
> Thanks for your help.
>
> Dr. Heike Grabsch, Germany
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 12:49:00 -0600
> From: Daisey Joseph <djoseph@impath.com>
> Subject: RE: Immuno controls for FNA's
>
> You can purchase breast carcinoma cell lines from American Type Culture
> Collection (ATCC)
> This organization warehouses cell lines that have been characterized in
the
> literature for many different uses i.e. molecular or immunocytochemical
etc.
> MCF7 is one cell line you can purchase from them that is ER+ and PR+.
Call
> 800-638-6597 in USA & Canada or 301-881-2600 outside the USA.
>
> - -----Original Message-----
> From:	Koutney, Janice [SMTP:JKoutney@cytometry.com]
> Sent:	Thursday, November 05, 1998 6:47 AM
> To:	'histonet@pathology.swmed.edu'
> Subject:	Immuno controls for FNA's
>
> Hi All,
> We have just been asked to start doing ER/PR on FNA slides.  My question
> is, what do we use as controls?  A  paraffin block does not seem to be
> an appropriate contol for a fluid.  We do not work in a hospital or a
> histology lab.  We are in a reference lab in which the already embedded
> blocks come to us for immuno staining.  We just cut the slides and
> perform the immunos, so access to FNA fluids or fresh tissues is not an
> option here.  We do have a Shandon Cytospin here.    Any ideas or
> comments will be most appreciated.
>
> Janice Koutny  HT(ASCP)
> Cytometry Asso.
> Brentwood, TN
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 14:00:51 -0600
> From: "Kellar, Eric" <kellarec@MSX.UPMC.EDU>
> Subject: RE: anti-EBA
>
> Cora:
>
> Sternberger Monoclonals, Inc.
> Alpha Center
> Hopkins Bayview Research Center
> 5210 East Ave.
> Baltimore MD 21224-2736
> 1-410-550-2644
>
> Eric Kellar
> Histology/Immunohistochemistry
> University of Pittsburgh Medical Center
>
> 	----------
> 	From:  Corazon Bucana [SMTP:bucana@audumla.mdacc.tmc.edu]
> 	Sent:  Thursday, November 05, 1998 12:04 PM
> 	To:  HistoNet@Pathology.swmed.edu
> 	Subject:  anti-EBA
>
> 	I would appreciate knowing the full address or phone number of
> Sternberger
> 	Monoclonals, Baltimore, MD.  A paper referenced this company as a
> source of
> 	antibody to a barrier protein.
>
> 	Thanks,
>
> 	Cora Bucana
> 	*******************************************************
> 	Corazon D. Bucana, Ph.D.
> 	Department of Cell Biology
> 	U.T. M.D. Anderson Cancer Center
> 	1515 Holcombe Blvd.  Box 173
> 	Houston, Texas 77030
> 	Phone: (713) 792-8106
> 	Email:bucana@audumla.mdacc.tmc.edu
> 	FAX: (713) 792-8747
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 14:15:24 -0600
> From: BCPCOMMUN@aol.com
> Subject: finger and toe nails- thank you
>
> Thank you for all the  helpful information. We are going through all the
ideas
> and will let you know what seems to work best for us.
>
> I'm new to the histonet and am enjoying it very much.
>
> Thanks,
> Carol
>
> (Carol Panken) bcpcommun@aol.com Grand Rapids,  MI
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 14:26:07 -0600
> From: Gayle Callis <uvsgc@msu.oscs.montana.edu>
> Subject: Marine animal fixation
>
> Humason, older edition, recommended sea water be used with the fixative,
> formaldehyde in your case.  Your marine animals will probably like it
> better since the salinity is important to maintain the isotonicity of
> the cells ((yours blow up!).  I guess on could say the seawater is a
buffer
> in itself.  Once fixed, I don't know if you need to increase salt in your
> immunostaining buffer.  This is is sometimes done with buffers, for IHC.

>
> There is an artificial sea water recipe in this book, but requires some
> interesting salts, etc to make it up.
>
> Gayle Callis
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 14:37:32 -0600
> From: oshel@terracom.net (Philip Oshel)
> Subject: Re: Halloween & Guy Fawkes
>
> This may be true. After all, England made a hero out of Scott, but seems
to
> ignore Shackleton.
>
> But then why don't you celebrate July 4th? 8-) (bloody colonials...)
>
> Phil
>
> >Seems to be a Brit mentality to celebrate failures, maybe it's because
we
> >don't have enough successes :-(.
> >I mean who else would make a hero out of someone who came last in a
sporting
> >arena, remember the fuss we made out of Eddie (the Eagle) Edwards.
> >Stan
> >-----Original Message-----
> >From: RUSS ALLISON <Allison@cardiff.ac.uk>
> >To: histonet@pathology.swmed.edu <histonet@pathology.swmed.edu>
> >Date: 05 November 1998 09:11
> >Subject: Halloween & Guy Fawkes
> >
> >
> >O.K., all you guys in the States make a big ball out of halloween -
> >of pagan origin, if I remember aright.
> >
> >Well, to day is OUR big day.
> >We celebrate Guy Fawkes - who FAILED to blow up Parliament - never
> >quite understood why we celebrate a failure?  Anyway, ever since, on
> >the 5th November, we have our own pagan festival whereby we all go to
> >great lengths to show our old hero Guy how he SHOULD have gone about
> >it.
> >Hence, this evening we will all light bonfires, eat hot-dogs and down
> >the occasional amber nectar whist letting off fireworks galore!  The
> >sky will be lit up with rockets and sparks, people will be full of
> >bonhomie, firemen will earn overtime, casualty departments will be
> >full, tomorrow the skin banks will be empty.
> >
> >Why the hell couldn't Guy Fawkes get it right, then we could
> >celebrate Halloween and not ever have to worry about 5 November?
> >Russ Allison, Wales
>
> ****be famous! send in a tech tip or question***
> Philip Oshel
> Technical Editor, Microscopy Today
> PO Box 620068
> Middleton, WI  53562
> (608) 833-2885
> oshel@terracom.net
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 14:50:02 -0600
> From: oshel@terracom.net (Philip Oshel)
> Subject: Re: Marine animal fixation
>
> I can second this. All of my EM work was on marine animals, and 0.2
micron
> filtered seawater was the best buffer to use. Only problem is that the
> formalin might precipitate if the water is cold, say 4 deg. C or lower.
> (This was lots of fun when making up seawater buffered formalin+glut
using
> water from the Ross Sea. -2 deg. C.)
>
> Phil
>
> >Humason, older edition, recommended sea water be used with the fixative,
> >formaldehyde in your case.  Your marine animals will probably like it
> >better since the salinity is important to maintain the isotonicity of
> >the cells ((yours blow up!).  I guess on could say the seawater is a
buffer
> >in itself.  Once fixed, I don't know if you need to increase salt in
your
> >immunostaining buffer.  This is is sometimes done with buffers, for IHC.
> >
> >There is an artificial sea water recipe in this book, but requires some
> >interesting salts, etc to make it up.
> >
> >Gayle Callis
>
> ****be famous! send in a tech tip or question***
> Philip Oshel
> Technical Editor, Microscopy Today
> PO Box 620068
> Middleton, WI  53562
> (608) 833-2885
> oshel@terracom.net
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 15:01:33 -0600
> From: seeleyc@biogenex.com
> Subject: Casein Information
>
>
>
>  Biogenex has a universal blocking reagent that is made up of casein,
> which is capable of reducing nonspecific background in
> immunohistochemistry. Feel free to contact us for anymore information.
>
> Thank You,
> BioGenex
> Technical Support
> 1-800 -421-4149
> Fax 925-866-2594
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 15:45:14 -0600
> From: Cheryl Crowder <crowder@vt8200.vetmed.lsu.edu>
> Subject: Artefacts
>
> Happy Guy Fawkes Day.  Any occasion to party is a good day.  But, a pagan
> holiday and be really fun!   Well, I have another mystery.  Our
Diagnostic
> Lab pathologists are complaining of an occasional artefact seen on some
of
> their tissues, not all and only rarely.  The area will be on the edge of
the
> tissue and appears to look like cautery marks, but these tissues have not
> been cauterized.  We have tried multiple things to narrow down the
problem
> but to no avail.  We added an extra alcohol after 70%, 80% and 95%,
reduced
> the freezing time before cutting, lowered the temperature of the paraffin
> and embedding center, decreased the time in the automatic stainer for
> drying.  The only other thing we ahven't done yet is check the rea in the
> automatic processor to see if there is a hot spot.  Has anyone had a
problem
> like this?  Have you noticed any hot spots in processors?  Any other
ideas?
> We are at a loss.
>         I will be out of the lab until Tuesday so if you can think about
it
> and let me know we would really appreciate the help.  Thank you.   Cheryl
> Crowder.
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 15:45:36 -0600
> From: "Hagerty, Marjorie A." <mhagerty@emc.org>
> Subject: Immuno Oven
>
>
> Hi Folks,
> A friend of one of the pathologists is looking to buy a new oven to
incubate
> immunos. Any recommendations? He wants to know a manufacturer and a model

> number.
> Thanks!
> Marg
> EMC, Rancho Mirage, CA
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 16:46:04 -0600
> From: SMITHS5@pfizer.com
> Subject: Re[2]: Halloween & Guy Fawkes
>
>      Yup, we sure do.  To be historically accurate, I suppose we should
>      hang, draw and quarter him first.  His target was James I ("the
wisest
>      fool in christendom"), not parliament, as the protestant James was
>      being hard on the Catholics (Fawkes and his co-conspirators were
>      Catholics).
>
>      Does anyone out there know the rest of the rhyme:
>
>      "Remember, remember the fifth of november
>      Gunpowder, treason and plot"?
>
>      I've forgotten it and it's bugging the life out of me
>
>
> ______________________________ Reply Separator
> _________________________________
> Subject: RE: Halloween & Guy Fawkes
> Author:  HistoNet@Pathology.swmed.edu at Internet
> Date:    11/5/98 12:10 PM
>
>
> Don't you burn Guy in effigy annually, as part of your bonfire?  (Just to

> punish him every year for his attempt?)
>
> > ----------
> > From:  RUSS ALLISON[SMTP:Allison@cardiff.ac.uk]
> > Sent:  Thursday, November 05, 1998 12:50 AM
> > To:  histonet@Pathology.swmed.edu
> > Subject:  Halloween & Guy Fawkes
> >
> > O.K., all you guys in the States make a big ball out of halloween -
> > of pagan origin, if I remember aright.
> >
> > Well, to day is OUR big day.
> > We celebrate Guy Fawkes - who FAILED to blow up Parliament - never
> > quite understood why we celebrate a failure?  Anyway, ever since, on
> > the 5th November, we have our own pagan festival whereby we all go to
> > great lengths to show our old hero Guy how he SHOULD have gone about
> > it.
> > Hence, this evening we will all light bonfires, eat hot-dogs and down
> > the occasional amber nectar whist letting off fireworks galore!  The
> > sky will be lit up with rockets and sparks, people will be full of
> > bonhomie, firemen will earn overtime, casualty departments will be
> > full, tomorrow the skin banks will be empty.
> >
> > Why the hell couldn't Guy Fawkes get it right, then we could
> > celebrate Halloween and not ever have to worry about 5 November?
> > Russ Allison, Wales
> >
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 16:46:34 -0600
> From: SMITHS5@pfizer.com
> Subject: Re[2]: Halloween & Guy Fawkes
>
>      Yup, we sure do.  To be historically accurate, I suppose we should
>      hang, draw and quarter him first.  His target was James I ("the
wisest
>      fool in christendom"), not parliament, as the protestant James was
>      being hard on the Catholics (Fawkes and his co-conspirators were
>      Catholics).
>
>      Does anyone out there know the rest of the rhyme:
>
>      "Remember, remember the fifth of november
>      Gunpowder, treason and plot"?
>
>      I've forgotten it and it's bugging the life out of me
>
>
> ______________________________ Reply Separator
> _________________________________
> Subject: RE: Halloween & Guy Fawkes
> Author:  HistoNet@Pathology.swmed.edu at Internet
> Date:    11/5/98 12:10 PM
>
>
> Don't you burn Guy in effigy annually, as part of your bonfire?  (Just to

> punish him every year for his attempt?)
>
> > ----------
> > From:  RUSS ALLISON[SMTP:Allison@cardiff.ac.uk]
> > Sent:  Thursday, November 05, 1998 12:50 AM
> > To:  histonet@Pathology.swmed.edu
> > Subject:  Halloween & Guy Fawkes
> >
> > O.K., all you guys in the States make a big ball out of halloween -
> > of pagan origin, if I remember aright.
> >
> > Well, to day is OUR big day.
> > We celebrate Guy Fawkes - who FAILED to blow up Parliament - never
> > quite understood why we celebrate a failure?  Anyway, ever since, on
> > the 5th November, we have our own pagan festival whereby we all go to
> > great lengths to show our old hero Guy how he SHOULD have gone about
> > it.
> > Hence, this evening we will all light bonfires, eat hot-dogs and down
> > the occasional amber nectar whist letting off fireworks galore!  The
> > sky will be lit up with rockets and sparks, people will be full of
> > bonhomie, firemen will earn overtime, casualty departments will be
> > full, tomorrow the skin banks will be empty.
> >
> > Why the hell couldn't Guy Fawkes get it right, then we could
> > celebrate Halloween and not ever have to worry about 5 November?
> > Russ Allison, Wales
> >
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 18:31:09 -0600
> From: "Mark & Carrie Byrne" <eire@teleport.com>
> Subject: RE: Immuno controls for FNA's
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 18:46:02 -0600
> From: "Mark & Carrie Byrne" <eire@teleport.com>
> Subject: glucose-glucose oxidase protocol
>
> i had such an overwhelming response asking for this reference i'm just
going
> to post it here for all.
>
> Andrew SM, Jasani B (1987) Histochem J   19:426-30
>
> best of luck to all who try it....i think it's a great protocol for use
on
> frozens.  it does take a bit of time....you incubate for 1hr. @ 37C.
>
> carrie kyle-byrne
> eire@teleport.com
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 18:55:15 -0600
> From: Bruce Abaloz <b.abaloz@zoology.unimelb.edu.au>
> Subject: Re:Cryostats!
>
> We purchased a motorized Leica CM3000 a few years back now & "cannot sing
its
> praises any higher"!!We are a teaching Facility & therefore have
all"levels of
>
> experience" so to speak, utilizing it....very User
Friendly...reliable.Has a
> separate specimen temp.control as well...down to -50Cin a matter of
minutes
> ..as it has 2 compressors...therefore chamber temp.& speci.temp.can be
> individually controlled.Easy to dismantle for cleaning..all stainless
steel
> etc.It has been supersceded I'm sure but well worth looking @ the new
> version.Cheers,Bruce in OZ.
>
> - --
> Bruce Abaloz
> HISTOLOGIST
> Department of ZOOLOGY	       *  ph:    +61 3 93446282
> The University Of Melbourne    *  fax:   +61 3 93447909
> Parkville Victoria.3052        *  email: b.abaloz@zoology.unimelb.edu.au

>
> AUSTRALIA.
>                     IF YOU WANT TO BE HAPPY......BE!!
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 19:45:20 -0600
> From: dave@biocare.net
> Subject: Casein
>
>
> I am the author of the original casein paper for immunohistochemistry.
> If you want the real stuff, contact me at Biocare Medical.
>
> David Tacha HTL (ASCP)
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 20:15:34 -0600
> From: mmcgee@medicine.adelaide.edu.au
> Subject: Re: EXAKT sectioning of s/steel
>
>
>      We are having difficulty sectioning bone specimens containing
>      stainless steel orthopaedic implants.  If anyone has had success
using
>      the EXAKT macro cutting device to do this, I would greatly
appreciate
>      any advice provided regarding the type of blade used and any other
>      important protocol details.
>
>
>
>      Marg McGee
>      Dept Orthopaedics and Trauma
>      Royal Adelaide Hospital
>      Sth Australia
>      Ph.  61-8-8222 5078
>      Fax: 61-8-8232 3065
>      email: mmcgee@medicine.adelaide.edu.au
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 20:45:16 -0600
> From: "Mark & Carrie Byrne" <eire@teleport.com>
> Subject: glucose-glucose oxidase protocol (again?)
>
> i'm posting this out again because i got an email saying the orig. wasn't
> sent for some "computer" reason.
>
> i had such an overwhelming response asking for this reference i'm just
going
> to post it here for all.
>
> Andrew SM, Jasani B (1987) Histochem J   19:426-30
>
> best of luck to all who try it....i think it's a great protocol for use
on
> frozens.  it does take a bit of time....you incubate for 1hr. @ 37C.
>
> Carrie Kyle-Byrne
> eire@teleport.com
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 22:45:31 -0600
> From: mmcgee@medicine.adelaide.edu.au
> Subject: Re: EXAKT sectioning of s/steel
>
>
>      We are having difficulty sectioning bone specimens containing
>      stainless steel orthopaedic implants.  If anyone has had success
using
>      the EXAKT macro cutting device to do this, I would greatly
appreciate
>      any advice provided regarding the type of blade used and any other
>      important protocol details.
>
>
>
>      Marg McGee
>      Dept Orthopaedics and Trauma
>      Royal Adelaide Hospital
>      Sth Australia
>      Ph.  61-8-8222 5078
>      Fax: 61-8-8232 3065
>      email: mmcgee@medicine.adelaide.edu.au
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Nov 1998 23:45:34 -0600
> From: Gerard Spoelstra <spoelstr@numbat.murdoch.edu.au>
> Subject: Silver Impregnation Method for the heart conduction system
>
> Hi Histonetters!
> Does anyone have access to the silver impregnation method for staining
the
> heart conduction system. A paper on this was published in the Japanese
> Anatomical Journal, Kaigogaku, 1992 , Vol67, No4, Pages 298-307 authored
by
> Morita,T and
> Shimada,T.
>
> Gerard Spoelstra
> Histopatology Laboratory
> School of Veterinary and Biomedical Science
> Murdoch University
> Western Australia
>
>
>
>
> Here are the messages received yesterday!