Re: Disposal of Hx with Hg, etc.
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
On Sun, 8 Nov 1998, B.A.Murray wrote:
> ... How do you dispose of your hematoxylin with mercuric oxide?
The mercuric oxide used to do the oxidizing hardly dissolves in
water (0.005 g per 100 ml) and the product of its reduction,
mercurous oxide, is even less soluble, even in acids. [Data
from Lange's Handbook of Chemistry] Consequently nearly all
the mercury used in making a Harris-type haemalum remains
in insoluble form. If you filter the product, the evil
mercury is in the filter paper. This can be set aside and
allowed to dry, making a very small object for safe disposal.
If you do not filter the matured staining solution, the
mercury will accumulate in a sludge at the bottom of the
bottle. If it's a small, cheap plastic bottle, it can be
sent for disposal. If it's a big, expensive glass-stoppered
vessel, you'll need to wash it out and collect the
poisonois sludge in filter paper.
The staining solution itself (with no insoluble material)
does not contain mercury. It can and should be used repeatedly
until its potency diminishes. Rinsing solutions are therefore
harmless too, because they do not contain mercury.
All this trouble with mercury and silly safety officers is
most easily avoided by not using the accursed element. Iodate
is traditional and a lot less toxic. All the standard textbooks
have mercury-free recipes for haemalum staining solutions.
> Another question,how many of you still make your Hematoxylin?
If you buy the solution you pay for the transportation of
water, glass and protective packing. A single small order
of cheap solid chemicals, all minimally hazardous, has to
be a big saving. Here in prosperous SW Ontario no academic
outfit (relying on research grants) could afford to buy
pre-made solutions of uncertain composition. A laboratory
with generous government or other external support might be
able to afford the luxury of saving one man-hour instead
of making enough staining solution to last a few months.
This doesn't answer your "how many" question, but it might
encourage others to make up their own routine alum-hematoxylin
solutions. You can do it if you can read, weigh and measure
volumes.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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