While I agree that Carnoy's fixed  tissue can go directly to 100% ETOH, (we
do a 95% step) it is important to keep in mind that while you may have used
absolute (100%) to make your Carnoy's it can no longer be considered 100%
ETOH as soon as you add anything (i.e. chloroform and acetic acid). In some
receipes this makes it a 60% alcohol solution. We use 15 minute changes of
alcohol and xylene for avian leg skin. Oh Oh, I think I may have opened
myself up for some fried chicken jokes. anita