Re: Carnoy's -Reply

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From:Bryan Llewellyn <> (by way of histonet)
To:histonet <>
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-----Original Message-----
From: M.A. Jennings <>
To: <>
Date: November 10, 1998 1:02 PM
Subject: Re: Carnoy's -Reply

>While I agree that Carnoy's fixed  tissue can go directly to 100% ETOH, (we
>do a 95% step) it is important to keep in mind that while you may have used
>absolute (100%) to make your Carnoy's it can no longer be considered 100%
>ETOH as soon as you add anything (i.e. chloroform and acetic acid). In some
>receipes this makes it a 60% alcohol solution. We use 15 minute changes of
>alcohol and xylene for avian leg skin. Oh Oh, I think I may have opened
>myself up for some fried chicken jokes. anita

But remember that Carnoys fluid contains NO water.  Both glacial acetic acid
and chloroform are anhydrous (or should be).  95% ethanol contains 5% water,
so going to it instead of anhydrous (100%) ethanol is a retrograde step and
only serves to increase processing time.

Bryan D. Llewellyn

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