RE: Superfrost slides

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From:"Kellar, Eric" <kellarec@MSX.UPMC.EDU> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Kathy:

The slides are manufactured for Allegiance by Erie Scientific. Customer
service 1-800-258-0834.

Eric Kellar
Histology/Immunohistochemistry
University of Pittsburgh Medical Center

	----------
	From:  KathY Liucci [SMTP:KLIUCCI@lhsnet.com]
	Sent:  Friday, November 06, 1998 1:04 PM
	To:  Histonet@Pathology.swmed.edu
	Subject:  Superfrost slides

	Hello:
	I was wondering if anyone has had this problem.  We have superfrost
plus slides, with the coating being pink.  We use these for special stains.
The slides are purchased from Allegiance but come with no label, so we do
not know who manufactures these slides.
	The problem is this:  our control slides are not staining well with
these pink slides.  We especially noticed this with our Masson trichrome and
our retic stains.  When we take the slides out of water, there is a sheeting
off of the water, like what happens when you go from alcohol that has some
water in it, into xylene.  So we did an experiment with putting the control
on our regular white, superfrost slides and did the same special stains.  We
noticed a big improvement.  They stained very well.  So after all this, my
question is, could it be possible that these slides are coated or charged
too much and are actually diminishing the staining?  Any information will be
appreciated.
	Thank you
	Kathy Liucci





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