Pam,
Is there also a problem with the Hematoxylin staining in those areas? If so,
my guess is that the deparaffinization is not complete. Also, are you drying
in the microwave? I have seen similar problems when dried in the microwave,
just a thought.
Hope you post the solution, this is very curious.
Mary Georger

> ----------
> From: 	Pam Plumlee[SMTP:PPlumlee@thermolase.com]
> Sent: 	Thursday, November 12, 1998 12:50 PM
> To: 	'histonet@pathology.swmed.edu'
> Subject: 	Manual H&E staining problem
>
> Histonetters: I do manual H&E staining on human and animal tissue.
> Lately some sections or parts of sections are not taking up the Eosin.
> This problem is very intermittent, but very noticeable on slide review.
> Sometimes the first level on a slide will stain great and on the third
> level the whole right half of the section will not stain.  I've tried
> drying the slides longer, alternating the side holder from side to side,
> to make sure the paraffin melts correctly.  Any ideas?  Thanks in
> advance, pplumlee@thermolase.com
>