We experienced the same problem and went back to using antigen retrieval.
Our results were some of the best Ki-67 staining we have seen.


Sharon


>>>>>Does anyone have a protocol for Ki-67 (Zymed or Immunotech antibodies)
>>>>>when tissue is fixed in Prefer?  Our Immuno's are done by automation.
>>>>>We do not antigen retrieve with prefer, but is there something else we
>>>>>can do?  We are getting all negative staining and I know some should
>>>>>be positive. Thanks
Kathy

Sharon B. Bledsoe
Indiana University
School of Medicine
Dept. of Surgery
Indianapolis, IN 46202
sbledsoe@iupui.ed