RE: Ki-67 Protocol

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From:sbledsoe <> (by way of histonet)
To:histonet <>
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We experienced the same problem and went back to using antigen retrieval.
Our results were some of the best Ki-67 staining we have seen.


>>>>>Does anyone have a protocol for Ki-67 (Zymed or Immunotech antibodies)
>>>>>when tissue is fixed in Prefer?  Our Immuno's are done by automation.
>>>>>We do not antigen retrieve with prefer, but is there something else we
>>>>>can do?  We are getting all negative staining and I know some should
>>>>>be positive. Thanks

Sharon B. Bledsoe
Indiana University
School of Medicine
Dept. of Surgery
Indianapolis, IN 46202

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