More...manual staining
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From: | Pam Plumlee <PPlumlee@thermolase.com> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
To: Nancy from Sakura and other helpful netters: My H2O bath is 45
degrees with DI h2O and 1 ml of Sta On. I use Superfrost slides. When
I pick up my sections, I lean them against my waterbath at an angle to
let them dry. I put them in a 60 degree oven for a least 1 hour. I use
Xylene times 3 for 5 minutes ea and !00% times 3 for 1 minute and 95%
times 2 for 1 minute for depariffinization. After bluing I use a tap
rinse and then a DI rinse for at least a minute. I also use a quick 80%
rinse before Eosin. I just found out my server will be down for a week
starting the 13th, then I go on vacation till Dec.!!! Thanks again for
all the suggestions...this is a great resource. Thanks, Pam
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