LacZ Staining

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Dear Histomembers and more particulary Anita,

I would like to perform lacZ staining, on whole mice embryos (between E14,5
and E18,5 ).
It seems that it is important to remove the skin to allow a goood
infiltration of the substrate.
what's about the time of incubation for lacZ staining.
Is overnight incubation enough? or more...
Could anyone suggest me something about it?



Ayadi Abdelkader
Institut de Genetique et de Biologie Moleculaire et Cellulaire (IGBMC)
BP 163
67404 Illkirch cedex FRANCE

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