Jennifer:
The Ab diluent is made of 1% bovine serum albumen in PBS with ).05% Tween 20 and 0.1% sodium azide.'NaCl is part of the PBS but I have never used or kbown anybody using additional NaCl
René J.
"Harvey, Jennifer Lynn" wrote:
Histonet,
Has anyone tried NaCl in there diluent buffer for IHC, if so what
concentration. I read about it in DAKO's immunostaining methods booklet
but they don't state the concentration. This is my last chance with my
background problem. I have a sticky collagen issue I cant get rid of.
Thanks!
Jennifer Harvey, HT(ASCP) QIHC
Vanderbilt Vision Research Center
RM 8105 MCE North Tower
1215 21st Ave. South
Nashville, TN 37232-8808
Phone: 615-936-1486
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