With the DAKO autostainer I used Melan-A at 1:50 x 30 min after HIER with citrate at pH6 with good results.
  René J.
  

"Miley, Joannah C."  wrote:
  Our lab is demoing a Dako immuonstainer and I am having a hard time
optimizing our Melan-A. The dilutions I have tried are 1:50, 1:100, and
1:200. I have tried antigen retreival with EDTA pH9 and Citrate buffer
at pH 6. I am incubating in the primary and polymer for 20 minutes
each. I am still having a lot of trouble with excessive muddy
background. Any ideas??

Thanks,

Joannah Miley, HT(ASCP)
NW Pathology
Bellingham, WA
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