Hello Joannah,
I use Dako's Melan-A on FFPE human tissue @ 1:50
I use their TRS for 40 minutes in a steamer and
incubate  for 15 minutes room temp. I then use
LSAB2, but I suspect that you'll get better results
with your polymer or Dako's polymer Env+ Mouse or Flex.

Good luck.


Dana Settembre, HT ASCP
Immunohistochemistry Lab
UMDNJ - University Hospital
Newark, NJ    USA


>>> "Miley, Joannah C."  11/28/07
1:31 PM >>>
Our lab is demoing a Dako immuonstainer and I am having a hard time
optimizing our Melan-A.  The dilutions I have tried are 1:50, 1:100,
and
1:200.  I have tried antigen retreival with EDTA pH9 and Citrate
buffer
at pH 6.  I am incubating in the primary and polymer for 20 minutes
each.  I am still having a lot of trouble with excessive muddy
background.  Any ideas??
 
Thanks,
 
Joannah Miley, HT(ASCP)
NW Pathology
Bellingham, WA
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