LabVision also has a pre-dilute for clone D5,which is what we use undiluted.
Bernice
Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D
Deltour
Sent: Monday, November 19, 2007 12:14 PM
To: 'Sate Hamza'; Histonet@lists.utsouthwestern.edu
Subject: RE: SPAM-LOW: Re: [Histonet] MiTF on a Ventana Immunostainer
Invitrogen-Zymed has a pre-dilute that can be used in a prep-kit on the XT.
This is the same one that I am currently troubleshooting.
Douglas D. Deltour HT(ASCP)
Histology Manager
Professional Pathology Services, PC
One Science Court
Suite 200
Columbia, SC 29203
Office (803)252-1913
Fax (803)254-3262
Doug@ppspath.com
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-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sate Hamza
Sent: Monday, November 19, 2007 12:23 PM
To: Douglas D Deltour; Histonet@lists.utsouthwestern.edu
Subject: SPAM-LOW: Re: [Histonet] MiTF on a Ventana Immunostainer
Thanks to all who have responded to my message.
I asked my colleague and she said that she has not ordered any antibody
yet. She said she wanted to see if she can get a protocol first before she
orders the antibody.
She was wondering what the source of your antibody was ..
Thanks again ..
Sate
On 11/19/07, Douglas D Deltour wrote:
>
> We just started using the MITF-1 antibody on the XT. I got it validated
> and
> we were running it for about 2 months and then all of a sudden it stopped
> working, but that is another story. Which company did your colleague get
> the
> antibody from? I used the same protocol that I used for the MART-1 for
> validation. This was working great for the MITF until.....
>
> [---]
>
>
>
>
--
Sate Hamza, MD, FRCPC
Dermatopathologist
Winnipeg, Canada
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