RE: [Histonet] how long does it take?

From:"Bernice Frederick"

We tell researchers a minimum of two weeks for any project involving IHC
since we have multiple clients- 1 week longer if titreing is involved. Some
times you get it right away,sometimes not.

Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611

-----Original Message-----
[] On Behalf Of louise
Sent: Wednesday, November 21, 2007 4:15 AM
Subject: [Histonet] how long does it take?

Dear Histonetters,

First off, let me say what a great experience the NSH symposium was! It was
great to finally meet y'all. Made me realise that I am not the only one
So here's my question rearding IHC.......
What is a reasonable work-up time for a new antibody? The parameters are:
1. Ab researh only, not used in the particular species of animal I am using
2. Ab titre needs to be assessed
3. not sure what a good control might be
4.Will be using archival tissue where fixation/processing may be
5. using a manual ABC/DAB technique
6. All buffers etc made up in-house
Any ideas??

Best regards

Louise Renton
Bone Research Unit
University of the Witwatersrand
South Africa
"There are nights when the wolves are silent and only the moon howls".
George Carlin
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