My original question was, how to get the best frozen lung-slides without
the use of liquid nitrogen or isopentan. - just with the cryocut, chucks
and OCT. The pathologist complains about "overfrozen" edges and a bad
overall morphology. I will ask him for more descriptionary details or
get a picture. Thank you for your hints.
But that's the thing I'm struggling with. Liquid nitrogen with or
without 'additives' is the best way of reducing ice artefact, so by not
using it I expect you to get worse results. If you want 'better'
morphology then post fix.
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