RE: [Histonet] TAZ protein and uses

From:"patsy ruegg"

Yes,. I do ihc all the time on antibodies listed for use on westerns and
elisa only.  Sometimes they work on ffpe tissue and sometimes they don't.
just establish testing criteria and do use the dilution recommendations for
elisa/westerns as a guide, in general I usually concentrate 10x for ihc over
elisa recommendations but you still have to do a titer range comparing
several dilutions and times, also you will need to try several AR
pretreatments, generally I use HIER with lph and hph buffer, I also try some
enzyme digestion proteinase k, pepsin, etc. until I get something that
works, the key is to have good positive control tissue to test.

Patsy Ruegg, HT(ASCP)QIHC
12635 Montview Blvd. #216
Aurora, CO 80010
fax 720-859-4110 
-----Original Message-----
[] On Behalf Of Gagnon, Eric
Sent: Thursday, November 22, 2007 10:02 AM
Subject: [Histonet] TAZ protein and uses

Has anyone tried immunohistochemistry using the TAZ-protein antibody?
(alternate names BTHS, CMD3A, EFE)
A researcher has asked us to try it on the Ventana Benchmark XT, even though
the product insert sheet lists Western Blot and ELISA as uses.  
Perhaps the larger question is: should IHC be attempted using antibodies
that do not list FFPE tissue as one of the antibody's uses?
Eric Gagnon MLT
Histology Laboratory
Kingston General Hospital
Kingston, Ontario, Canada

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