Hi Abby. First, Histogel is great! We published a paper in the Journal
of Histotechnology, Neuropath special issue, in June. It deals with
using Histogel for CNS samples, but can totally be used as a reference
for other tissues.

For tissues (not cell suspensions) I recommend placing the tissue in a
Peel Away mold, squirt molten histogel on the tissue and cool the block
(refrig).  We have completely eliminated the use of "T-bags" for minute
specimens.  For larger fragments of brain tumor, we use a larger base
mold and just add a little histogel to "hold the fragments" together. It
will reduce tissue shrinkage and will preserve the orientation.

This is the reader's digest version. If you have additional questions,
don't hesitate to call or email me.
Have a great day.
Jennifer

Jennifer L. Hofecker, HT(ASCP)
Vanderbilt University Medical Center
Division of Neuropathology 
Nashville, TN
ph. (615)343-0083
fax. (615)343-7089
NSH Quality Control Committee Chair
 
 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abigail
M. Butler
Sent: Wednesday, November 21, 2007 9:13 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Histogel Question


I completely understand how to use histogel to embed free floating
cells. But how would I embed a piece of tissue that obviously is not
placed in a tube? I am considering purchasing Histogel for the first
time and have many ideas of how I could use it, just not sure the proper
steps to take to embed let's say two mice eyes. Thanks Abbie Butler, HT
(ASCP), QIHC University of Georgia College of Veterinary Medicine

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