Does anybody have experience staining rat brain for the substance P receptor? Which company would you recommend? I have also read that many researchers fix tissue with paraformaldehyde and picric acid? Unfortunately, when we designed our experiments we did not anticipate that we would have to stain our tissue with this antibody and I was wondering how important is it to fix tissue with a paraformaldehyde/picric acid solution. Our rats were perfused with physiological saline followed by 4% paraformaldehyde. Brains were removed and postfixed for 72 hrs in the same solution, before being placed in 0.1% sodium azide/PB solution prior to sectioning on a Vibratome at 50 micron.
Thanks for the help,
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