[Histonet] how long does it take?

From:"louise renton"

Dear Histonetters,

First off, let me say what a great experience the NSH symposium was! It was
great to finally meet y'all. Made me realise that I am not the only one
So here's my question rearding IHC.......
What is a reasonable work-up time for a new antibody? The parameters are:
1. Ab researh only, not used in the particular species of animal I am using
2. Ab titre needs to be assessed
3. not sure what a good control might be
4.Will be using archival tissue where fixation/processing may be
5. using a manual ABC/DAB technique
6. All buffers etc made up in-house
Any ideas??

Best regards

Louise Renton
Bone Research Unit
University of the Witwatersrand
South Africa
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George Carlin
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