Hi Nick, I wasn't very successful at finding a CD19 Ab for mouse FFPE but I did find a PAX5 Ab that worked for detecting B cells, it came from Lab Vision (RB-9406), it should give nuclear staining in B cells but not in plasma cells though. You could potentially try CD79a for that as I think that comes up strong in plasma cells but again getting a good Ab for mouse FFPE for that is tricky.
Deeley Research Centre
Date: Mon, 26 Nov 2007 11:48:41 -0500
From: "Madary, Joseph"
Subject: [Histonet] need cd19 help in mouse spleens and a job
Content-Type: text/plain; charset="us-ascii"
I need help finding a source and even a decent procedure that works on
cd19 on FFPE mouse spleens. I have a decent procedure for cd3 and cd45,
but the path wants cd19, so I figured I would throw it to the experts
first. Thanks in advance. Also Medimmune is looking for a histotech
here in Rockville Md. Someone with GLP, frozen and IHC experience.
Nick Madary, HT/HTL(ASCP)QIHC
Histology Mgr, Medimmune
"To the extent this electronic communication or any of its attachments
contain information that is not in the public domain, such information
is considered by MedImmune to be confidential and proprietary, and
expected to be used only by the individual(s) for whom it is intended.
If you have received this electronic communication in error, please
reply to the sender advising of the error in transmission and delete the
original message and any accompanying documents from your system
immediately, without copying, reviewing or otherwise using them for any
purpose. Thank you for your cooperation."
Histonet mailing list
End of Histonet Digest, Vol 48, Issue 36